HPLC测定兔血浆烟酰水杨酸及其活性代谢产物水杨酸和烟酸浓度  

HPLC Determination of Nicotinylsalicylic Acid and Its Active Metabolites Salicylic Acid and Nicotinic Acid in Rabbit Plasma

作　　者：张梅[1] 吴越[1] 杨光[1] 周园[1] 李丽燕[1] 丁一上[1] 

机构地区：[1]石河子大学药物研究中心,新疆石河子832002

出　　处：《石河子大学学报（自然科学版）》2005年第5期568-571,共4页Journal of Shihezi University(Natural Science)

基　　金：国家重点科技项目(攻关)计划(96-901-01-70)

摘　　要：本文采用血浆样品酸化后用乙醚萃取,高效液相色谱法测定,色谱柱为C18-ODS柱(5mm×150mm),柱温为30℃,流动相为甲醇-0.01%磷酸水溶液(45:55),流速1.0mL/min,苯巴比妥作为内标,检测波长230nm,按内标法定量测定,标准曲线在2～100μg/mL(烟酰水杨酸)、3～150μg/mL(水杨酸、烟酸)内,样品都有良好的线性(r都在0.9990以上),方法回收率在98.4%～108.9%,日内RSD小于5.0%,日间RSD小于5.4%.新建立的HPLC测定兔血浆中的烟酰水杨酸及其活性代谢产物水杨酸和烟酸浓度的方法简便快速,准确,灵敏度高,为进一步研究烟酰水杨酸体内代谢动力学提供测定方法.To study the pharmacokinetics of nicotinylsalicylic acid in rabbit, the HPLC method was established for separation and quantitative determination of nicotinylsalicylic acid in rabbit plasma. Nicotinylsalicylic acid and its metabolites nicotinic acid and salicylic acid in rabbit plasma were extracted by ether. The sample was separated on C18- ODS column （5 mm × 150mm）, with methanol-water with 0.01% H3PO4 （ 45：55 ） as the mobile phase （ flow rate 1.0mL/ min）, column temperature was 30℃ and detection wavelength was 230nm. Calibration curves were good linear within the concentration range of 2 - 100μg/mL for nicotinylsalicylic acid, 3 - 150μg/mL for nicotinic acid and salicylic acid in rabbit plasma. Recoveries of assay were 98.4% - 108.9% and relative standard deviations for inter-day and intra-days were less than 5.0% and 5.4% respectively.The HPLC method was found to be simple, rapid, sensitive and accurate.

关 键 词：烟酰水杨酸 水杨酸 烟酸 HPLC 血药浓度 

分 类 号：R97[医药卫生—药品]