一种提取和纯化肌钙蛋白C的新方法  

New method for purification of troponin C

作　　者：盖晓东[1] 丛进阳[1] 沈忠耀[1] 

机构地区：[1]清华大学化学工程系,北京100084

出　　处：《清华大学学报（自然科学版）》1996年第6期72-76,共5页Journal of Tsinghua University(Science and Technology)

摘　　要：肌钙蛋白Ⅰ是肌钙蛋白的一个亚基，它有３种异构体：快、慢骨骼肌肌钙蛋白Ⅰ和心肌肌钙蛋白Ⅰ。由于心肌肌钙蛋白Ⅰ具有极强的心脏特异性，因此引起众多学者的兴趣，研究检测患者血清内心肌肌钙蛋白Ⅰ的含量，来诊断急性心肌梗塞。利用肌钙蛋白Ｃ和肌钙蛋白Ⅰ的亲和层析来提取和纯化肌钙蛋白Ⅰ。建立了一种简单的肌钙蛋白Ｃ的提取和纯化方法，从兔肌１００ｇ中可提取肌钙蛋白Ｃ ７５ｍｇ，不仅高于以往研究结果（６０ｍｇ／１００ｇ兔肌），而且使实验周期缩短和实验操作大为简化。Troponin Ⅰ (Tn Ⅰ ) is the inhibitory subunit of troponin. Three isoforms of Tn Ⅰ have been identified: two skeletal Tn Ⅰ isoforms (fast and slow) and one cardiac Tn Ⅰ isoform. Cardiac Tn Ⅰ is suggested to be used for diagnosis of acute myocardial infarction (AMI) by detecting the content of cardiac Tn Ⅰ in patient's serum because of its specificity for AMI. Cardiac and skeletal Tn Ⅰ were prepared by affinity chromatography using immobilized troponin C (Tn C) in Sepharose-4B as matrix, relying upon the properties of specific Tn C-Tn Ⅰ binding in the presence of calcium. In this paper a new method for purification of Tn C has been introduced with yield of Tn C up to 75 mg/100g rabbit muscle , which is higher than those reported by previous authors. The purification process was much shortened and the procedures were simplified. The preparation of immobilized Tn C Sepharose-4B gel and the purification of Tn Ⅰ were also discussed in detail.

关 键 词：亲和层析 肌钙蛋白C 提取 提纯 

分 类 号：Q510.3[生物学—生物化学]