肢体火器伤神经体液髓鞘碱性蛋白释放表达实验研究  

Study on Neurohumoral Myelin Basic Protein Expression in Extremities Firearm Wound

作　　者：王庆松 陈曼娥 琚亚非 

出　　处：《中华创伤杂志》1996年第3期187-189,共3页Chinese Journal of Trauma

摘　　要：目的：探讨肢体火器伤中枢神经系统（ＣＮＳ）应变内环境紊乱的病理生理基础．方法：借助犬双后肢低、高速投射物伤模型，采用ＲＮＡ斑点杂交、ＥＬＩＳＡ法，动态观察、定量分析脑组织、脑脊液（ＣＳＦ）、血清髓鞘碱性蛋白（ＭＢＰ）表达与释放．结果：伤后８小时血清、ＣＳＦ、脑组织ＭＢＰ含量增高（Ｐ＜０．０１），高速组尤甚；低速组下丘脑，高速组下丘脑、海马区ＭＢＰ－ｍＲＮＡ表达增强（Ｐ＜０．０５）．结论：肢体火器伤虽未直接伤及大脑，但提示下丘脑、海马区受损，可能为ＣＮＳ应变反应的重要病理生理基础之一．Aim: To explore pathophysiological bases of disturbance of central nervous system (CNS) internal environment. Methods: On the basis of low and high velocity projectiles injury to thighs in dogs, ELISA, RNA dot blot hybridization was used to dynamically observe and quantitatively analyse the expression of myelin basic protein (MBP) in brain tissues, cerebrospinal fluid (CSF) and serum. Results: The levels of MBP in CSF, serum and brain homogenate at 8h posttrauma markedly increased ( P <0.01),especially in the high speed group; the expression of MBP mRNA in hypothalamus in the low speed group, and hypothalamus and hippocampus formation in the high speed group was significantly enhanced ( P <0.05). Conclusion: Hypothalamus and hippocampus formation were damaged inspite of no direct injury to the head in extremities firearm wound, which may play a consistent pathophysiological role in CNS stress reaction.

关 键 词：肢体 火器伤 中枢神经系统 髓鞘碱性蛋白 

分 类 号：R826.5[医药卫生—临床医学]