生物芯片技术难点和展望  

Technical Difficulties and Prospects of Biochip

作　　者：程波[1,2] 郭善利[1] 

机构地区：[1]聊城大学生命科学学院 [2]山东师范大学生命科学学院,山东济南250014

出　　处：《聊城大学学报（自然科学版）》2005年第4期57-60,70,共5页Journal of Liaocheng University:Natural Science Edition

摘　　要：简单回顾了生物芯片(Biochip)发展历史和基本定义,生物芯片是以预先设计的方式将大量的生物讯息密码(寡核苷酸、cDNA、基因组DNA、蛋白质等)固定在玻片、硅片等固相载体上组成的密集分子阵列.生物芯片技术本质是生物信号的平行分析,它利用核酸分子杂交、蛋白质分子亲和原理,通过荧光标记技术检测杂交或亲和与否,再经过计算机分析处理可迅速获得所需信息.本文主要概述了生物芯片技术的最新研究进展,并对生物芯片技术面临的挑战以及未来的发展方向作了讨论.Firstly reviewed the development history and basic definitions of biochip simply,biochip is a molecular microarray ,which is composed of some biological message codes （oligonucleotides ,cDNA, genome DNA, protein etc. ）arranged on solid surfaces due to a engineering design in advance. The essence of biochip technology is a parallel analysis for biology message. It is based on the principle of nucleic molecule hybridization or protein immunochemistry. The occurrence of hybridization or immunoreaction is detected by fluo-labelled technology. After analyzed by special computer program, the needed message can be obtained quickly. This paper summarizes the new advances in biochip technology , and talk about challenges and trends of this technology.

关 键 词：生物芯片 技术难点 展望 

分 类 号：Q503[生物学—生物化学] TQ460.5[化学工程—制药化工]