抗人Ⅳ型胶原蛋白单克隆抗体的制备和鉴定  

Generation and Characterization of Monoclonal Antibodies against Human Collagen Type 4

作　　者：莫畏[1] 窦良军 蒋盘宏[1] 

机构地区：[1]北京化学试剂研究所,北京市生化试剂高技术实验室

出　　处：《细胞与分子免疫学杂志》1996年第2期1-3,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：用人Ⅳ型胶原蛋白免疫ＢＡＬＢ／ｃ小鼠，克隆了一组分泌抗人Ⅳ型胶原蛋白的杂交瘤细胞株，并且对其中的Ａ２、Ａ４、Ａ５、Ｂ３和Ｅ２进行了鉴定。其抗体亚类分别为ＩｇＧ１、ＩｇＧ２、ＩｇＧ２ｂ、ＩｇＧ２和ＩｇＧ１。其亲和力常数Ｋａｆｆ＝６．６×１０８Ｍ（－１）～６．０×１０（１０）Ｍ（－１）。它们均能特异性地识别人Ⅳ型胶原蛋白。Hybridomas secreting McAbs to human collagen type Ⅳ were established by immunization of BALB/c mice with human collagen type Ⅳ (Ⅳ-CL). Five of these McAbs A2, A4,A5, B3 and E2 were well characterized and the Ig class and subclass were determined as IgG1,IgG2, IgG2b, IgG2 and IgG1 respectively. These McAbs reacted strongly with Ⅳ-CL moleculeswith the affinity constants(Kaff) of 6.6×108M-1～6.0×1010M-1 and did not have cross-reactivity with other types of collagen and irrelevant proteins.

关 键 词：单克隆抗体 Ⅳ型胶原蛋白 制备 肝纤维化 诊断 

分 类 号：R392－33[医药卫生—免疫学] R446.6[医药卫生—基础医学]