血清脂蛋白（a）酶联免疫吸附测定方法及正常参考值  

ENZYME-LINKED IMMUNOSORBENT ASSAY OF SERUM LIPOPROTEIN(A)AND ITS NORMAL REFRENCE VALUE

作　　者：谢玉才[1] 丁怀翌[1] 印彤[1] 蔡伟菁[1] 吴春芳[1] 龚兰生[1] 

机构地区：[1]上海第二医科大学附属瑞金医院心血管内科,上海第二医科大学

出　　处：《上海医学》1996年第3期128-131,共4页Shanghai Medical Journal

摘　　要：应用自制脂蛋白（ａ）单克隆抗体建立了双单抗夹心酶联免疫吸附测定法。本法特异性强，批内变异系数２．６％，批间８．１％，检测下限０．３ｍｇ／ｄｌ，回收率９８．６％，本法与ＴｉｎｔＥｌｉｚｅＬｐ（ａ）ｋｉｔ标准曲线形态相似，两者测值（ｎ＝８０）相关系数为０．８４８。检测３５９名正常人血清脂蛋白（ａ）水平为２２．３２±１９．７ｌｍｇ／ｄｌ，呈偏态分布。double antibody enzyme-linked immunosorbent assay for lipoprotein(a)[Lp(a)]inhuman serum has been established,using self-made monoclonal antibodies. The assayshowed no interference with low density lipoprotein,apolipoprotein B and plasminogen. within-run coefficients of variations(CVs)was 2.6%,and between-run CVs was 8.1%. The sensitivity was 0.3mg/dl;The Lp(a)levels of 80 samples by our ELISA were in good a-greement with those by Tint Elize Lp(a)(Biopool AB,Sweden)(r=0.848),and the stan-dard curves of the two were also similar. Using our ELISA,we quantified Lp(a)in normal and some patients. The reference va-lue was 22.32±19.77mg/dl,and the results showed that the valles were 29.28±27.45,30. 75±29.07,28.78±26.98 and 28.15±22.75mg/dl in patients with myocardial infarction,cerebral stroke,renal failure and diabetes mellitus,respectively.

关 键 词：脂蛋白 ELISA 血清 

分 类 号：R446.112[医药卫生—诊断学]