神经降压素对实验性神经细胞内兴奋性氨基酸含量影响的研究  

THE EFFECTS OF NEUROTENSIN AND ARGININE-VASOPRESSIN ON ASPARTIC AND GLUTAMIC ACIDS IN EXPERIMENTAL NEURONS

作　　者：孙晓江[1] 蔡琰[1] 苏敏[1] 王珺[1] 张毅[1] 邓锦波[1] 孙九伶[1] 高燕军[1] 

机构地区：[1]上海第二医科大学附属仁济医院神经生物学实验室,承德医学院附属医院神经生物学实验室

出　　处：《上海医学》1996年第3期132-134,共3页Shanghai Medical Journal

基　　金：上海高等院校科学发展基金和河北省自然科学基金

摘　　要：本研究采用小鼠神经母细胞瘤细胞（ＮＢＡ２）无血清培养建立神经细胞实验研究模型。以氨基酸自动分析术观察神经降压素（ＮＴ）对实验性神经细胞内氨基酸类神经递质的影响。结果发现：ＮＴ可使细胞内天门冬氨酸（Ａｓｐ）和谷氨酸（Ｇｌｕ）这两种兴奋性氨基酸含量显著降低（Ｐ＜０．０１），而对抑制性氨基酸中的甘氨酸（Ｇｌｙ）和γ－氨基丁酸（ＧＡＢＡ）的影响不大（Ｐ＜０．０５）。结果提示：ＮＴ使兴奋性氨基酸Ａｓｐ和Ｇｌｕ含量降低的作用可能是其延缓实验性神经细胞老化的机制之一。erum-free culture of mouse neuroblastoma A2(NBA2)cells was used in the experimen-tal study of neurons. High speed amino acid analyzer was used to observe the effects of neu-rotensin(NT)and on amino acid neurotransmitters contents in experimental neurons. After addition of NT separately into the culture medium,the following results were ob-tained:NT caused a decrease in the levels of aspartic(Asp)and glutamic(Glu)acids(p<0. 01),with no significant influence on GABA and glycin. The results suggested that theseneuropeptides may play an important role in the modulation of Asp and Glu levels in cellsduring experimental aging.

关 键 词：神经降压素 神经细胞 天门冬氨酸 谷氨酸 

分 类 号：R972.4[医药卫生—药品]