银杏叶提取物对局灶性脑缺血再灌注后线粒体损伤的保护作用  

The protective effect of Xinhani on mitochondria after focal brain ischemia-reperfusion in rats

作　　者：沈立[1] 薛雅静[1] 沈伏 张文奇[1] 张金荣[1] 李雅琴[1] 

机构地区：[1]哈励逊国际和平医院,河北衡水053000 [2]河北省卫生监督局

出　　处：《中国基层医药》2005年第12期1742-1744,共3页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的研究杏花雨注射液对大鼠局灶性脑缺血再灌注后脑线粒体中SOD、ATPase、MDA的影响,探讨其抗脑缺血作用的机制。方法雄性SD大鼠63只,随机分为9组(n=7)。假手术组、4个缺血对照组(缺血2 h分别再灌注0.5、1、24、h组,其中缺血2 h再灌注4 h设为模型组),3个杏花雨注射液(A)治疗组(高、中、低剂量组),金纳多注射液(B)治疗对照组。治疗组分别于术后立即舌下静脉注射A 5、10、20 mg/kg,B 10 mg/kg。测定脑线粒体中SOD、ATPase活性和MDA含量、基质游离钙浓度。结果局灶性脑缺血再灌注后脑线粒体中MDA含量、基质游离钙浓度明显升高(P<0.01),SOD、ATPase活性明显下降(P<0.01);A与B均能明显提高脑线粒体中SOD、ATPase活性(P<0.01),减少MDA、游离钙(P<0.01);两种注射液同等剂量差异无显著意义(P>0.05)。结论局灶性脑缺血再灌注后线粒体钙超载,自由基生成增加,杏花雨注射液可提高缺血再灌注大鼠脑线粒体总抗氧化活力,降低MDA、游离钙含量,保护缺血再灌注引起的神经元损伤。Objective To study the efiects of Xinhani solution on the concentraion of free Ca^2＋ ,SOD,ATPase, MDA in the brain mitochondria of rats during cerebral ischemia and reperfusion. Methods Sixty three sprague-dawley（SD） rats were divided randomly into nine groups： sham control group, injected intraperitoneally saline; Ischemia-reperfusion groups, including I（ischemia） 2h/R（reperfusion） 0.5,1,2,4h groups（ I2h/P4h is vehicle group） saline ： Ginaton（B） 10mg/kg treatment groups ; Xinhani （A） 5,10,20mg/kg treatment groups. A, B were injected through sublingual vein of rats at the start of ischemia. The activities of SOD, ATPase and contents of MDA,concentration of free Ca^2＋ in the brain mitochondria of rats were determined. Results The brain mitochondria was injured remardably after postischemia reperfusion. Increasing of content of MDA, free Ca^2＋ （P 〈 0.01 ）, decreasing of the activity of SOD, ATPase （ P〈 0.01 ） in mirochondria showed the injury of mitochondria. Compared vehicle to treatment groups,A and B injection could dose-dependently increase activities of SOD and ATPase（P 〈 0.01 ） and decrease contents of MDA,frec Ca^2＋ （P 〈 0.01 ）. There was no significant difference between A 10mg/ kg groups and B 10mg/kg groups（P 〉 0.05）. Conclusion The results show that Xinhani injection solution could protect brain from ischemia-reperfusion injury with increase vitality to resist oxidation of brain tissue and decrease content of MDA.

关 键 词：再灌注损伤 基质游离钙 丙二醛 超氧化物歧化酶 腺苷三磷酸双磷酸酶 动物实验 银杏叶提取物 局灶性脑缺血 杏花雨注射液 脑线粒体 

分 类 号：R285.5[医药卫生—中药学]