破壁蜂花粉对蛋鸡免疫功能及肠道菌群的影响  

Impact of Broken Wall Bee Pollen on Immune Function and Intestinal Bacterium Group of Chickens

作　　者：王允超[1] 李桂杰[2] 路建彪[1] 朱瑞良[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]山东宝来利来生物工程股份有限公司,山东泰安271000

出　　处：《家畜生态学报》2005年第6期17-21,共5页Journal of Domestic Animal Ecology

基　　金：山东省优秀中青年科学家科研奖励基金(03BS140)资助

摘　　要：试验将槐花蜂花粉进行振动磨超微粉碎破壁,分别按1 g/kg、5 g/kg、10 g/kg三个不同剂量混到蛋鸡饲料中,从7日龄开始饲喂至50日龄,每个剂量分两个试验,试验一用新城疫Ⅳ系疫苗免疫雏鸡,利用β-微量法监测血清中的血凝抑制抗体效价的动态变化;试验二用大肠杆菌油乳苗免疫雏鸡,利用间接血凝法监测血清中大肠杆菌抗体效价的动态变化。分别定期称重捕杀取脾、胸腺、法氏囊,计算免疫器官指数,取肠道内容物计数大肠杆菌和乳酸菌,测定肠道内pH值。结果表明,不同剂量的破壁蜂花粉均能不同程度地提高病毒抗原性抗体效价和菌体抗原性抗体效价,对雏鸡免疫器官的发育以及肠道内环境、菌群的平衡也有一定影响。In the experiment, the wall of Scholar tree bee pollen was broken by vibromill and attrition,and was mixed and fed in the fodder to 50 days chickens by 1g/kg,5g/kg, 10g/kg,in three different dosage separately. Each dosage was divided into two tests. Test one use ND-Ⅳ vaccome immune chick, through β the micro-law to determine the dynamic change of the blood in the serum and congeal which suppresses the antibody titer. Test two use colom bacillus oil to assist the pharmaceutical vaccine shoot immune chick,through indirect blood congeal law to determine the dynamic change of serum of colon bacillus antibody titer. The two tests respectively involved. Regular weighing,catching. Killing and fetching the spleen,chest gland and bursa of fabricius, calculating the immune organ index,fetching intestinal content thing to count colon bacillus and lactic acid bacteria as wellas, determining pH value in the intestines. The result indicates that the different dosage of broken wall bee pollen can improve the virus antige of antibody titer and bacteria body antigen of antibaly titer in rarious degree, intestines environment, bacteria balance of group have a certain influence on growht of immune organ of chickens.

关 键 词：破壁蜂花粉 蛋鸡 抗体效价 免疫器官指数 

分 类 号：S811.5[农业科学—畜牧学]