妊高征患者胎盘组织中胎盘异铁蛋白表达的研究  

Study on the expression of placental isoferritin in the placenta of pregnancy induced hypertension syndrome.

作　　者：张瑞霞[1] 相文佩[1] 

机构地区：[1]山东省胶南市人民医院妇产科,266400

出　　处：《中国优生与遗传杂志》2006年第1期62-63,66,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的观察胎盘异铁蛋白(p lacental isoferritin,PLF)在妊高征(pregnancy induced hypertension syndrom e,PIH)患者胎盘组织中的表达,探讨其在血管病变中的作用。方法HE染色观察胎盘绒毛血管病变;免疫组化染色法检测妊高征胎盘组织(妊高征组)和正常妊娠胎盘组织(正常组)中PLF的表达,通过高清晰度彩色病理图文分析系统对其定量分析;W estern b lot检测胎盘血管组织中PLF蛋白质的表达。结果HE染色可见妊高征组胎盘绒毛细小血管壁增厚,管壁纤维素样坏死发生率显著高于正常组(P<0.01);免疫组化染色定量分析发现,PLF在中、重度妊高征胎盘组织中的表达明显低于轻度妊高征组和正常组(P<0.01);W estern b lot显示两组胎盘组织中均有34kDa的PLF蛋白质表达,但PIH组表达量明显降低(P<0.01)。结论妊高征患者胎盘组织中PLF表达水平降低参与了妊高征患者胎盘绒毛血管的病变过程。Objective ： To investigate the expression of placental isoferritin （PLF） in the placenta of the patients with pregnancy induced hypertension syndrome （PIH） and discuss its role in the pathogenesis of PIH. Methods： The pathology changes in placental vessel were observed by HE staining; Immunohistochemistry was used to detect the expression of PLF in placental tissue on patients with PIH （PIH group） and normal pregnant women （normal group）, HPIAS （High Resolution Pathological Image Analysis System） -1000 was used to determine the quantity of PLF; Western blot analysis was applied to detect the expression of PLF. Results： The thickening, fibronoid necrosis of placental vessel in PIH were significantly higher than those in normal group; Immunohistochemistry analysis tested that the levels of PLF expressions on moderate and severe PIH patients were significantly lower than that of normal group （ P 〈 0. 01 ） ; Western blot analysis showed that both groups express 34kDa PLF protein but the level is significantly lower in women with PIH than in the normal group （ P 〈 0. 01 ） ; Conclusion ： The level of PLF expression in PIH decreases in placenta of women with PIH, indicating that these may be involved in the pathogenesis of PIH.

关 键 词：PLF 胎盘 妊娠并发症 心血管 

分 类 号：R714.246[医药卫生—妇产科学]