速冻和缓慢冷冻法对精子运动特征的影响  

The effect of cryopreservative methods on motility parameters of human spermatozoa

作　　者：刘睿智[1] 孙妍[1] 许宗革[1] 刘嫒[1] 江露[1] 

机构地区：[1]吉林大学基础医学院细胞生物学教研室,长春130021

出　　处：《中国男科学杂志》2006年第1期8-10,共3页Chinese Journal of Andrology

基　　金：吉林省科技厅资助项目(20030433)

摘　　要：目的了解冷冻方法对人精子运动特征的影响。方法精液标本进行速冻和缓慢冷冻保存,应用计算机精液分析仪进行精子运动特征分析。结果冷冻复温后精子运动能力与冷冻前精子运动能力比较明显下降(P<0.001,P<0.05);速冻与缓慢冷冻方法保存的精子运动参数相比较差异均无显著性(P>0.05)。结论冷冻保存易导致精子运动能力下降,速冻与缓慢冷冻方法对精子运动参数影响无明显差异。Objective To study the effect of cryopreservative methods on motility parameters of human spermatozoa. Methods Human semen sample were cryopreserved by fast frozen and slow frozen method. The sperm motility parameters were analyzed by computer assisted semen analyzer （CASA）. Results Post-thawing sperm motility was significantly lower than that before （P 〈 0.001, P 〈 0.05）, whereas there were no statistics differences （P 〉 0.05） in sperm motility between fast frozen and slow frozen in GYC and glycerol groups. Conclusion Cryopreservation induces the descent of sperm motility. The velocity of cryopreservation could not affect sperm motility.

关 键 词：精了 低温保存 精子运动 

分 类 号：R318.52[医药卫生—生物医学工程]