c-jun对离体睾丸间质细胞睾酮分泌作用的机理研究  

The effects and mechanism of c-jun on testosterone secretion in isolated rat Leydig cells

作　　者：袁双虎[1] 于金明[1] 于甬华[1] 杨新华[1] 刘连珍[1] 李延持[1] 徐斯凡[2] 

机构地区：[1]山东省肿瘤医院放疗科,济南250117 [2]中央民族大学生命与环境科学学院

出　　处：《中国男科学杂志》2006年第1期13-16,共4页Chinese Journal of Andrology

摘　　要：目的本研究拟通过c-jun反义寡脱氧核苷酸(ASODNs)观察c-jun在调节hCG促进睾丸间质细胞(leydigcells,LC)睾酮分泌中的作用机制。方法用c-junASODNs拮抗c-jun,再加用cAMP观察其对睾酮分泌的影响,用放射免疫方法检测睾酮水平。结果hCG可刺激LC睾酮分泌,是LC功能研究的有用模型。c-junASODNs呈剂量依赖性地抑制hCG诱导下的离体LC的睾酮分泌(P<0.01)。加用cAMP后睾酮分泌增加。结论c-jun促进hCG诱导的大鼠LC的睾酮分泌,c-jun表达可能与cAMP相关。Objectives To investigate the effects and mechanism of c-jun on hCG induced testosterone secretion in isolated rat Leydig cells （LC） by anfisense oligodeoxynucleotides （ASODNs）. Methods hCG was used to induce the testosterone secretion of LC ,c-jun ASODNs were used to antagonise the effects of c-jun, then cAMP were added and testosterone was measured by radioimmunoassay. Results The testosterone secretion of LC in vitro could be induced by hCG, which was a good model for the functional study of LC. c-jun ASODNs decreased hCG-induced testosterone secretion of LC in a dose-dependent manner and cAMP increased the testosterone secretion （P〈0.01）. Conclusion It is suggested that c-jun proto-oncogene enhanced the testosterone secretion of LC and the expression of c-jun is possibly connected with cAMP.

关 键 词：莱迪希细胞 基因 jun 寡脱氧核苷酸 反义 睾酮 CAMP 

分 类 号：R335[医药卫生—人体生理学]