药物高通量亲合色谱筛选中定性定量模型的建立  

Qualitative and Quantitative Models for High ThroughputScreening of Drugs Based on Affinity Chromatography

作　　者：刘白玲[1] 李松军[1] 

机构地区：[1]中国科学院成都有机化学研究所,成都610041

出　　处：《中国科学院研究生院学报》2006年第1期31-38,共8页Journal of the Graduate School of the Chinese Academy of Sciences

基　　金：国家自然科学基金项目(20574071)资助

摘　　要：通过对AC高通量筛选过程的分析,建立了AC技术筛选药物的定性及定量模型,为高通量药物筛选提供了必要的理论指导.The affinity chromatography （AC） technique,based on the affinity principle of ligand and receptor, as well as the specificity and reversibility of their combination, is a considerably efficient technique for high throughput screening（HTS） of drugs. Since AC shows obviously the dynamical characters, associating and dissociating between ligands and receptors present in its column, it can present much more information than other related techniques. The information contained by its eluting curve reveals directly the interaction of ligand-target and its quantitative characterization, thereby facilitating drug discovery. Based on the analysis of AC-based HTS, the present study establishes a series of qualitative and quantitative models. Because of their usability, these models are normally expected to play a positive role in the future for guiding the process of drug screening.

关 键 词：高通量药物筛选 亲合色谱筛选法 筛选原理与过程 定性定量模型 

分 类 号：R965.1[医药卫生—药理学]