抗环瓜氨酸肽抗体及相关自身抗体在诊断类风湿性关节炎中的意义  

Significance of anti-cyclic citrullinated peptide antibody and other correlated antibodies in the diagnosis of rheumatoid arthritis

作　　者：张利方[1] 闫有功[1] 付丽萍[1] 钟情[1] 

机构地区：[1]广州军区武汉总医院检验科,湖北武汉430070

出　　处：《检验医学》2006年第1期12-14,共3页Laboratory Medicine

摘　　要：目的探讨抗环瓜氨酸肽(CCP)抗体对类风湿性关节炎(RA)诊断的意义,并比较与抗核周因子抗体(APF)、抗角蛋白抗体(AKA)、类风湿因子(RF)之间的相关性以及在临床诊断上的意义。方法对119例RA患者血清和183例对照者血清用酶联免疫吸附试验(ELISA)以及人类颊黏膜和大鼠的食道角质层为底物的间接免疫荧光法、散射速率比浊法进行检测。结果119例RA患者抗CCP抗体敏感度和特异度分别为49.6%和97.3%,与非RA对照组和正常对照组比较差异有显著性(P<0.05)。抗CCP抗体与APF、AKA、RF重叠阳性率为41.2%、36.1%、和43.6%,经统计学分析,抗CCP抗体与APF、AKA、RF之间存在相关性,但并不完全重叠。结论抗CCP抗体将是协助诊断RA的一个新的实验指标,能提高RA的早期诊断率。Objective To investigate the significance of anti-cyclic eitrullinated peptide （CCP） antibody in the diagnosis of rheumatoid arthritis （RA） and compare the relativity of CCP with anti perinuelear factor （APF） ,anti keratin antibody （AKA）and rheumatoid factor （RF） . Methods Serum samples from 119 RA patients and 183 normal control subjects were tested by enzyme-linked immunosorbent assay （ELISA） （the coated antigen CCP was synthesized from its known eDNA sequence） ,indirect immunofluoreseenee and rate nephelometry. Results The sensitivity and specificity of anti-CCP in the 199 RA patients were 49.6 % and 97.3 % respectively, there was significant difference between test group and control group（P 〈0.05 ）. The cross positive rate of anti-CCP with APF, AKA , RF were 41.2 % ,36.1% and 43.6% respectively. It was demonstrated that there was a relativity between anti-CCP, APF, AKA, RF. Conclusions The anti-CCP assay has high sensitivity and good specificity and might be used as a valuable index in the early diagnosis of RA.

关 键 词：抗环瓜氨酸肽 抗体 类风湿性关节炎 酶联免疫吸附试验 

分 类 号：R446.62[医药卫生—诊断学]