液体单试剂酶法测定血清碳酸氢根  

Single reagent enzymatic method for determination of serum bicarbonate ion

作　　者：陈洁[1] 顾国宝[1] 华俊[1] 卢燕[1] 李静[1] 

机构地区：[1]上海市闸北区中心医院检验科,上海200070

出　　处：《检验医学》2006年第1期52-54,共3页Laboratory Medicine

摘　　要：目的建立一种稳定的单试剂酶法测定血清碳酸氢根（HCO3^-）。方法利用自配单试剂建立了两点法的检测方法，可满足全自动和手工测定，用Bayer 1650生化仪对本方法进行方法学评价。结果本法的线性范围可达50mmol／L，批内变异系数（CV）为2．48％，批间CV为3．24％，平均回收率102．8％，与血气分析仪（X）相关良好，Y=1．019X-0．745，r=0．9903，黄疸、脂血、溶血标本对结果几乎无影响，健康人静脉血HCO3^-浓度为21．5～32．3mmol／L。结论本法操作简便、反应特异、灵敏准确、试剂较稳定，适用临床检测。Objective To establish a stable single reagent enzymatic method for determination of serum bicarbonate ion. Methods The two-point assay was established by self-made single reagent which could satisfy automatic and manual operations. Bayer 1650 chemistry analyzer was used to evaluate the assay. Results The linear range was up to 50 mmol/L. The intraassay CV and interassay CV were 2.48% and 3.24% respectively. Average recovery rate was 102.8%. The correlation was good with blood gas analysis, Y = 1. 019X - 0. 745,r = 0.990 3. Icteric, hemolytic and lipidic samples showed almost no interference. The serum bicarbonate ion levels of healthy persons ranged from 21.5 mmol/L to 32.3 mmol/L. Conclusions The method is simple, specific, sensitive, and stable. It is suitable to be used for clinical analysis.

关 键 词：碳酸氢根 酶速率法 单试剂 

分 类 号：Q592.1[生物学—生物化学]