繁枝蜈蚣藻粗提物体外抗单纯疱疹病毒研究  

Study on the effects of amylose extracted from Grateloupia filicina against herpes simplex virus in vitro

作　　者：朱艳梅[1] 王一飞[1] 张美英[1] 朱良[1] 岑颖洲[2] 

机构地区：[1]暨南大学生物医药研究开发基地,广州510632 [2]暨南大学化学系,广州510632

出　　处：《中国卫生检验杂志》2006年第1期6-8,共3页Chinese Journal of Health Laboratory Technology

基　　金：广东省自然科学基金团队项目(粤科基办[2003]11号)(2004E039213);广东省科技攻关项目(国际合作)(2003C50301);广州市科技攻关项目(2005Z3-E0411)

摘　　要：目的:研究海藻繁枝蜈蚣藻抗单纯疱疹病毒Ⅰ型活性。方法:采用空斑减数实验测定5种不同提取方法所得蜈蚣藻粗提物抗HSV-1活性,计算空斑抑制率和ED50,并从药物对细胞的保护、对病毒增殖的影响及对病毒感染细胞的综合作用3个方面初探繁枝蜈蚣藻抗单纯疱疹病毒活性机理。结果:蜈蚣藻多糖能明显抑制HSV-1的致病变作用,ED50依次为8.72、12.16、8.93、8.49和805 982.62μg/m l,药物在对细胞的保护及对病毒感染细胞的综合作用中均表现出明显活性。结论:繁枝蜈蚣藻有显著的抗HSV-1活性,且初步推测蜈蚣藻多糖抗HSV-1活性是作用在病毒和受体结合、侵入Vero细胞阶段。Objective：To investigate the activities of anti - Herpes simplex Ⅰ virus of amyloses axtracted from Grateloupiafilicina via five kinds of extraction methods. Methods：HSV - 1 was inoculated on Vero cells, Amylase axtracted from Grateloupiafilicina was added to the medium in various concentrations, after 72 hours incubation, plaques were counted and the 50% effective dose （ED50） was calculated in plaque reduction assay. Initial studies on antivirus mechanism in three aspects： the drug protective action, the drug influence on virus proliferation and the drug synthesis action on virus were studied. Results：Amyloses had conspicuous anti - Herpes simplex Ⅰ virus activities, their ED50 were 8.72, 12. 16, 8.93, 8.49 and 805 982. 62μg/ml, respectively. Amyloses had antivirus activities in the drug protective action and the drug synthesis action on virus, however, had not in the drug influence on virus proliferation, Conclusion： Gratelottpia filicina has conspicuous anti -Herpes simplex Ⅰ virus activity, and the activity happened in the stage of HSV - 1 binding, adsorpting with Vero cell.

关 键 词：繁枝蜈蚣藻 细胞病变效应法(CPE) MTT 空斑抑制率 

分 类 号：R373.11[医药卫生—病原生物学]