HIV流行毒株的DNA macroarray基因分型技术的研究  

Determination of the epidemic HIV strain genotypes by DNA macroarray

作　　者：倪崖[1] 谭卓 房国坚[1] 金妙坚 李坤[1] 蒋娴 黄云坚 

机构地区：[1]浙江省医学科学院,杭州310013 [2]浙江澳亚生物工程研究院,杭州310018

出　　处：《中国卫生检验杂志》2006年第1期12-14,共3页Chinese Journal of Health Laboratory Technology

基　　金：浙江省医药卫生科学研究基金项目(2002B001);浙江省科研院所专项研究开发项目(2005F13024)

摘　　要：目的:建立一种特异、相对简便的H IV流行毒株基因分型新技术。方法:从H IV-1抗体阳性标本中提取H IV前病毒DNA,使用H IV-1型共用引物进行PCR扩增。同时设计一条型特异性的探针,然后通过制作DNA m acroarray芯片,鉴别样本的H IV亚型。结果:DNA m acroarray分析可以检测我国H IV主要流行株基因型的特异性序列:B、C、CRF01-AE和CRF07-BC。结论:DNA m acroarray方法对H IV亚型进行分析是可行的,可以在H IV流行病学调查中应用。Objectlve：To establish a specific, relatively simple new technique in determining the epidemic HIV strain genotypes, nethods：Proviral DNA extracted from the HIV - 1 positive specimen was amplified by PCR with the shared primer of HIV - 1 type. The specific probe which could determinate the epidemic HIV strain in our country was designed in the primer. Then HIV subtypes were determined after making DNA macroarray. Results： The analysis could determinate the specific sequences of the epidemic HIV strain genotypes in our country： B, C, CRF01 - AE and CRF07 - BC. Conclusion： DNA macroarray was available for the determination of HIV subtypes completely and could be applied to the investigation of epidemiology.

关 键 词：人类免疫缺陷病毒 DNA MACROARRAY 基因分型 

分 类 号：R512.91[医药卫生—内科学]