金丝桃提取物对DNA损伤的保护作用  

A Study of the Protective Effect of the Extract of Hypericum Sinense L.against DNA Injury

作　　者：杨瑜[1] 项光亚[2] 阮金兰[2] 

机构地区：[1]武汉大学中南医院药剂科,430071 [2]华中科技大学同济医学院药学院,武汉430030

出　　处：《医药导报》2006年第2期107-109,共3页Herald of Medicine

摘　　要：目的观察金丝桃提取物对DNA损伤的保护作用。方法配制硫酸铜-邻啡罗啉反应体系,制备DNA损伤模型体系,在该模型体系中分别加入金丝桃3个化学成分部位(F002、F003、F004)的甲醇提取物,每个化学成分部位提取物设5个不同浓度,观察金丝桃3个不同化学成分部位提取物对损伤DNA化学发光动力学曲线的影响。结果随着金丝桃各化学成分部位提取物的加入,DNA损伤发光动力学曲线峰明显下降且后移;且提取物浓度越高,发光动力学曲线峰下降越明显。金丝桃3个不同化学成分部位的抗氧化能力强弱顺序为F002>F004>F003。结论金丝桃属于预防型和断链型抗氧剂,对DNA损伤有明显保护作用,而且这种作用与化合物的极性有关。Objective To study the protective effect of the extract of Hypericurn sinense L. against DNA injuries. Methods A cupric sulfate-reaction system and a DNA-injury model system were prepared. Methanol extracts of the 3 chemical component parts of Hypericum sinense L. F002, F003 and F004 were separately added to the systems. 5 different concentrations of each of the 3 chemical component parts were used in the experiment. A chemiluminescence method was used to study the effects of the extracts of the 3 different chemical component parts on the chemiluminescence dynamic curves of the injured DNA. Results A striking fall and backward shifting of the peaks of the chemiluminescence dynamic curves of the injured DNA were demonstrated with the addition of the extracts of the different chemical component parts of Hypericum sinense L. , and the higher the concentration of the extracts, the grater the fall of the peaks of the chemiluminescence dynamic curves of the injured DNA. The sequence of the strength of the antioxidative capability of the 3 components was shown to be F002 〉 F004 〉 F003. Conclusion Hypericum sinense L. belongs to preventive and chain-breaking antioxidants. It has definite protective effects against DNA injury and the effects are related to the polarity of the chemical compounds.

关 键 词：金丝桃提取物 DNA损伤 保护作用 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]