双黄连粉针剂体外抗HIV-1作用的实验研究  

Experimental Study on the Influence of Shuanghuanglian Injection against HIV-1 in vitro

作　　者：曾祥凤[1] 曾耀英[1] 李海仙[1] 林长乐[1] 李莉平[1] 

机构地区：[1]暨南大学组织移植与免疫教育部重点实验室,广东广州510632

出　　处：《河南中医学院学报》2006年第1期3-5,共3页Journal of Henan University of Chinese Medicine

基　　金：国家"十五"科技攻关计划中医药治疗艾滋病专项基金(编号:2004BA719A09-03)

摘　　要：目的:探讨双黄连粉针剂在体外是否有抗HIV作用及其可能的作用机制。方法:采用荧光染料Calcien AM标记HIV感染的慢性H9细胞(H9/HIV-1ⅢB)与不同稀释浓度的双黄连粉针剂作用后,与靶细胞MT-2细胞混合培养,观察MT-2和H9/HIV-1ⅢB融合细胞的变化;MTT法检测双黄连对HIV感染细胞的保护作用;HIV-1 P24抗原试剂盒检测药物作用后的细胞培养上清P24抗原含量的变化。结果:双黄连粉针剂能够抑制MT2和H9/HIV-1ⅢB融合,能够保护HIV感染细胞的死亡,减少HIV-1 P24抗原的表达。结论:双黄连粉针剂在体外具有抗HIV-1的作用。Objective： To investigate the inhibitory effects of Shuanghuanglian （SHL） injection on human immunodeficiency virus type 1 （HIV- 1） infection in vitro and the possible mechanisms. Methods： Calcein- AM stained H9/HIV- 1Ⅲ B cells were treated with serially diluted SIlL injection, then mixed with target ceils MT - 2, HIV - 1 mediated cell fusion was observed under fluorescent microscopy. The protecting effect of SHL on HIV - 1 - infected cells was determined by MTT. Viral replication was evaluated by mea suring the levels of p24 antigen in culture supematants by ELISA, Results： SHL injection could inhibit fusion of MT- 2 and H9/HIV 1Ⅲ B ceils, could protect HIV linfected ceils from death, and decrease p24 antigen release. Conclusion： SHL injection can reduce HIV- 1 infection in vitro.

关 键 词：双黄连 HIV-1 细胞融合 钙黄绿素AM 

分 类 号：R512.91[医药卫生—内科学] R373.9[医药卫生—临床医学]