蓝藻氢代谢相关酶及其分子生物学研究进展  

Progress in Molecular Biology of Enzymes Involved in the Hydrogen Production of Cyanobacteria

作　　者：张学成[1] 张晓辉[1] 徐涤[1] 

机构地区：[1]中国海洋大学海洋生物工程系,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2006年第1期19-25,共7页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(30471317)资助

摘　　要：综述了蓝藻中催化氢代谢反应的2种关键酶—固氮酶和氢化酶的生物学特征、分子基础及其编码基因和转录等最新研究进展。蓝藻在固氮细胞中的净产氢量是固氮酶和氢化酶(包括吸收氢化酶和双向氢化酶)综合作用的结果,但在非固氮情况下的产氢则主要是由双向氢化酶催化的。可通过有效利用基因工程技术对产氢相关酶基因进行改造,改进生物产氢系统。Energy crisis causes the hot spot on biohydrogen production. Here we summarized the current progress of nitrogenase and hydrogenase in cyanobacteria, including the biological character and molecular composition of the enzymes as well as their genes and the transcription regulation. In cyanobacteria, the net production of hydrogen in nitrogen-fixing heterocysts is determined by the integrative effects of nitrogenase and hydrogenase （composed by uptake hydrogenase and hi-directional hydrogenase）, while in the non-nitrogen-fixing cells, the hydrogen is produced mainly by the hi-directional hydrogenase. So the biohydrogen production system in cyanobacteria can be improved by genetic engineering technique.

关 键 词：生物产氢 蓝藻 固氮酶 氢化酶 

分 类 号：Q949.22[生物学—植物学] Q783