栉孔扇贝、华贵栉孔扇贝及其种间杂交子代、种内交配子代的ISSR分析  

ISSR Analysis of Two Species of Scallop(Chlamys farreri,C.nobilis) and Their Intra-And Inter-Species Mating Descendants

作　　者：刘广绪[1] 包振民[1] 胡景杰[1] 王师[1] 姚冰[1] 战爱斌[1] 

机构地区：[1]中国海洋大学生命科学与技术学部,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2006年第1期71-75,共5页Periodical of Ocean University of China

基　　金：国家高技术研究发展计划资助(2001AA620106;2003AA603022);国家重点基础研究发展规划项目计划(G1999012009);山东省优秀中青年科学家科研奖励基金(01BS10)资助

摘　　要：通过对栉孔扇贝、华贵栉孔扇贝及其两者的种间杂交子代、种内交配子代的ISSR标记分析,研究了4种子代群体之间以及子代群体与共享亲本个体间的遗传关系。使用3组ISSR引物,得到清晰的扩增位点169个,平均多态位点检出率66.44%。分析结果显示:杂交子代个体中具有来自父母本的扩增位点,表明杂交子代确为杂种;杂交子代群体不仅具有较高的遗传多样性,而且在与亲本个体和其他子代群体间的遗传关系上表现出与种内交配子代的明显差异;另外杂交子代与2种亲本的遗传关系上并不对等,表现出明显的倾向性,更加偏向于母本一方。Inter-Simple Sequence Repeats （ISSR） was applied to analyse the genetic relationship between Chlarnys farreri, Chlarnys nobilis and their four kinds of descendants in order to reveal the genetic differences and the genetic relationships between the parents and their descendants. Three ISSR primers were used to amplify the genome DNA, 169 bands were yielded and the average frequency of the polymorphism accoutered for 66.44%. The hybrids inherited bands from both parents, indicating that the hybrids were real heterogenous. By the analysis of the genetic diversity, genetic distance and the loci distributing trend, it was found that the hybrids not only showed higher genetic diversity, but also showed distinct difference from the inbreeding descendants in the genetic relationships with the parents or other descendants. And it was also found that the genetic relationships between hybrids and their parents were not equal, the female parents transfer red more genetic loci to their descendants, which indicated that hybrids inherited more genetic information from the female parents or less genetic information from the female parents had changed.

关 键 词：ISSR 栉孔扇贝 华贵栉孔扇贝 杂交子代 共享亲本 

分 类 号：Q321.2[生物学—遗传学] S968.3[农业科学—水产养殖]