肌苷对新生鼠缺氧缺血性脑病脑组织血管内皮生长因子、环氧合酶-2表达的影响  

Influence of Insoine on Expressions of Vascular Endothelial Growth Factor and Cyclooxygenase-2 in Newborn Rat with Hypoxic-Ischemic Encephalopathy

作　　者：高彬昌[1] 李琴 栾红[1] 

机构地区：[1]青岛大学医学院附属海慈医院儿科,山东青岛266033 [2]荣成市第二人民医院神经内科,山东荣成264309

出　　处：《实用儿科临床杂志》2006年第2期81-83,共3页Journal of Applied Clinical Pediatrics

摘　　要：目的探讨肌苷对尊重鼠缺氧缺血性脑病（HIE）脑组织血管内皮生长因子（VEGF）和环氧合酶-2（COX-2）表达的影响。方法将66只7日龄大鼠随机分为假手术组，对照组和肌苷组。通过结扎有颈动脉并缺氧2h，制备尊重鼠急性HIE模型。术前1d肌苷组予肌苷注射液，假手术组和对照组予相同剂量生理盐水，在给定的时间内取标本，通过免疫组织化学方法测定脑组织VEGF，COX-2的表达。结果假手术组脑组织无VEGF，COX-2阳性表达。对照组和肌苷组HI 2h后皮质和纹状体区部分神经元细胞呈阳性反应，之后阳性表达反应增强，12-24h达高峰后逐渐减少，至7d时只有少数细胞呈阳性表达。与同时间点对照组相比，肌苷组VEGF，COX-2蛋白表达均明显增强（P均〈0.01）。结论 肌苷可上调COX-2，VEGF蛋白的表达，可能是对脑缺氧缺血保护作用的途径之一。Objective To observe the expressions of vascular endothelial growth faetor（VEGF） and cyclooxygenase-2（COX - 2） in the cerebral tissue following hypoxic-ischemic encephalopathy（HIE） in newborn rats and explore the machanism of inosine in protecting against hypoxic- ischemic brain damage（HIBD）. Methods Sixty six newborn rats aged 7 days were divided into sham, control and experimental groups. HIE models were made by clamping the right cervical artery and making hypoxia for 2 hours. The rats in experimental group began injecting inosine, at 1 day before experiment, and the rats in the sham and control groups saline solution with same dose. The samples were made at the given time, and expressions of VEGF and COX - 2 were investigated by immunohistochemical technique. Results The cerebral tissue had no expression of VEGF and COX-2 in sham group. From 2 hours on cortex and striatum after HIE in control and experimental groups, expressions of VEGF and COX - 2 increased rapidly, peaking at 12 - 24 hours, and then decreased gradually, Expressions of VEGF and COX- 2 were higher in experimental group（All P〈0.01 ）, compared with control group at the same time. Conclusions Inosine can up - regulate expressions of VEGF and COX - 2 gene. It may be one of the machanisms in protecting against HIBD.

关 键 词：肌苷 脑缺氧 脑缺血 血管内皮生长因子 环氧合酶-2 

分 类 号：R722.12[医药卫生—儿科]