HIV-2gp105重组疫苗联合免疫引起小鼠更强的免疫应答  

Higher immune response inflicted by combined immunization of HIV-2 gp105

作　　者：李子健[1] 金宁一[1] 张立树[1] 江文正[2] 张洪勇[1] 李昌[1] 

机构地区：[1]军事医学科学院军事兽医研究所全军基因工程重点实验室 [2]华东师范大学生命科学学院,上海200433

出　　处：《吉林大学学报（医学版）》2006年第1期1-3,共3页Journal of Jilin University：Medicine Edition

基　　金：国家高技术研究发展计划项目(863计划)资助课题(2003AA219051)吉林省科技厅科技发展计划项目资助课题(20030550-1)

摘　　要：目的:探讨应用prime—boost免疫策略进行HIV-2外膜蛋白重组鸡痘病毒与重组DNA疫苗联合免疫引起小鼠的免疫应答。方法:将HIV-2 gp105重组DNA疫苗与重组鸡痘病毒以prime-boost免疫策略,免疫 BALB／c小鼠,检测免疫小鼠脾CD4+、CD8+T细胞亚群的数量、脾特异性CTL杀伤活性和血清抗体滴度。结果:重组DNA疫苗和重组鸡痘病毒prime-boost免疫策略和两种疫苗单独免疫均刺激小鼠产生针对HIV-2靶细胞的特异性CTL杀伤活性及特异性抗体,联合免疫组特异性CTL杀伤活性及特异性抗体水平高于单独免疫组(P<0．05)。结论:以HIV-2 gp105重组DNA疫苗进行基础免疫、以HIV-2 gp105重组鸡痘病毒进行加强免疫的prime-boost免疫策略能诱导小鼠产生更强的特异性细胞和体液免疫应答。Objective To provide fundamental data for developing an effective immunization strategy by different types of recombinant vaccines to protect HIV-2 infection. Methods Using prime-boost immunization strategy, BALB/c mice were inoculated muscularly with HIV-2 gpl05 recombinant DNA vaccine and fowlpox virus. The numbers of CD4＋ and CD8＋ subgroup of spleen T lymphocyte, the specific killing activities of spleen CTL and the titers of serum antibodies of the immunized mice were detected. Results HIV-2 specific target-killing activity of spleen CTL and serum HIV-2 specific antibody level were higher in combined immunization group than those in single immunization groups （P〈0. 05）. Conclusion The strategy of prime-boost may stimulate stronger specific celluar and humoral immune responses in mice.

关 键 词：HIV-2I gp105 疫苗 DNA 重组鸡痘病毒 免疫策略 

分 类 号：R392.11[医药卫生—免疫学]