中草药远志对醋酸铅诱发的小鼠精子非程序DNA合成的抑制作用  

Inhibitory effect of Poly gala tenyi folia willd on unscheduled DNA synthesis induced by Pb (CH_3COO)_2 in spermatogod of mice

作　　者：庞慧民[1] 高久春[2] 朱玉琢[1] 张丽娇[2] 

机构地区：[1]吉林大学基础医学院医学遗传学教研室,吉林长春130021 [2]吉林大学基础医学院细胞生物学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2006年第1期82-83,共2页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研发展计划项目资助课题(20010529)

摘　　要：目的:观察中草药远志对雄性小鼠生殖细胞遗传物质损伤的防护作用。方法:采用雄性小鼠生殖细胞非程序DNA合成实验。结果:远志各剂量组诱导的非程序DNA合成与正常对照组比较差异无显著性(P> 0．5);1．0～4．0 g·kg-1剂量远志对醋酸铅所诱导的非程序DNA合成有明显抑制作用(P<0．01),并且随着远志浓度的增加,其抑制作用逐渐增强,超过一定剂量,其抑制作用不再增强。结论:远志对小鼠生殖细胞遗传物质具有保护作用。Objeotive To observe the preventive effect of Polygala tenyifolia willd on damage of genetic materials in male mouse reproductive cells. Methods Unscheduled DNA synthesis （UDS） test in male mouse reperoductive cells was used. Results There was no significant difference （P~〉 0. 05） while UDS was induced by Polygala tenyifolia willd with various doses comparing with normal control. Nevertheless, Polygala tenyifolia willd in doses ranged from 1.0 to 4.0 g · kg^-1 inhibited strikingly UDS induced by Pb （CH3COO）2 （P〈0.01）, and the inhibitory effect was enhanced with the incresse of the concentration. However the effect did not change when the dose reached a certain concentration. Gonolusion Polygala tenyifolia willd shows a protective effect, namely antimutagenesis, on injury of genetic materials in reproductive cells of male mouse.

关 键 词：远志/药理学 醋酸铅 非程序DNA合成 生殖细胞 

分 类 号：Q343[生物学—遗传学] R282[医药卫生—中药学]