水稻抗纹枯病突变体的离体筛选  

Screening of Rice Somatic Mutants Resistant to Rice Sheath Blight in vitro

作　　者：敖世恩[1] 杨媚[1] 周而勋[1] 唐倩菲[1] 潘汝谦[1] 

机构地区：[1]华南农业大学植物病理学系,广东广州510642

出　　处：《华南农业大学学报》2006年第1期47-50,共4页Journal of South China Agricultural University

基　　金：国家自然科学基金(39870438);广东省自然科学基金(950383)

摘　　要：以水稻成熟胚培养产生的愈伤组织和悬浮细胞为筛选材料,以广东省水稻纹枯病菌Rhizoctonia solaniKühn强致病力菌株GD-11和GD-118产生的粗毒素为选择压力,从水稻品种的选择、粗毒素致病力的鉴定、突变体的筛选方法、突变体的再生以及突变体再生植株对粗毒素的抗病性鉴定等方面展开研究,建立了水稻抗纹枯病突变体的筛选体系并获得了抗病突变体.筛选体系为:两步筛选法,即以粤香占的愈伤组织和悬浮细胞为筛选材料,以φ(粗毒素)为3.5%和4.0%分别作为第1、2步的处理浓度,每一步处理的时间为20 d;筛选出的突变体再生苗的病情指数为37.55,而原始型再生苗的病情指数为87.50,抗病效果达57.08%.Rice somatic mutants resistant to rice sheath blight were screened in vitro with calli and suspension cells derived from rice mature embryo culture and by using pathogen crude toxin extracted from the culture filtrates of strong virulent isolates GD-11 and GD-118 of rice sheath blight pathogen Rhizoctonia solani Kühn colleted from Guangdong Province. In this thesis, studies were carried out on the choice of rice cultivars, virulence identification of crude toxin on detached rice leaves, mutant screening methods, regeneration of mutant plant, and resistance identification of regenerative plant to crude toxin. Thus, the system of in vitro screening somatic mutants for rice sheath blight resistance was established, and the mutants resistant to rice sheath blight were gained through this study. The screening system is two-step method, i. e. resistant somatic mutants were screened with calli and suspension cells of rice cuhivar Yuexiangzhan and by using pathogen crude toxin concentration of 3.5% and 4.0% for the first- and second-step respectively, the treatment time for each step was 20 days ; The disease index of regenerative plant of mutant was 37.55, while that of regenerative plant of original rice was 87.50, the effect of resistance was 57.08%,

关 键 词：水稻纹枯病 水稻纹枯病菌 粗毒素 抗病突变体 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]