香蕉穿孔线虫培养技术及其种群繁殖力研究  

Studies on Cultural Technique and Reproduction Fitness of Radopholus similis

作　　者：陈淳[1] 谢辉[1] 蔚应俊[1] 韩玉春[1] 秦丹[1] 黄春晓[1] 唐炯[1] 

机构地区：[1]华南农业大学植物线虫研究室

出　　处：《华南农业大学学报》2006年第1期61-64,共4页Journal of South China Agricultural University

基　　金：国家自然科学基金(30471163);农业部重点项目(农财发2003-9农财发2004-27);广东省科技计划项目(2004B20501001);广东省农业厅项目(粤财农2003-247粤财农2004-230)

摘　　要：以10 g/L琼脂粉培养基为对照,研究筛选了新的培养基.新筛选的100 g/L马蹄粉培养基、100 g/L玉米粉培养基和100 g/L菱角粉培养基可以培养用于繁殖香蕉穿孔线虫的胡萝卜愈伤组织.其中100 g/L马蹄粉培养基和100 g/L玉米粉培养基培养的胡萝卜愈伤组织繁殖香蕉穿孔线虫的效果较好,而100 g/L马蹄粉培养基培养周期短、速度快、成本低,可以用于代替琼脂粉培养基培养胡萝卜愈伤组织来繁殖香蕉穿孔线虫.香蕉穿孔线虫不同种群的繁殖力存在明显差异,本研究的10个种群中,来源于红掌的RH1种群的繁殖力最强.The culture media of 100 g/L chinese water chestnut powder, 100 g,/L corn powder and 100 g/L water chestnut powder were screened to used to culture carrot callus culturing Radophoolus sirnilis. Among these culture media, the culture results of 100 g/L chinese water chestnut powder and 100 g/L corn powder were preferable. The medium of 100 g/L chinese water chestnut powder had a good characteristics with short culture period, fast speed growth and lower costs, and suitable to replace the medium of agar powder. The reproduction fitness of 10 populations of R. sirniIis from different hosts and origins were compared in carrot disk cultures at 25 ℃. The result indicated that all R. similes populations completed their life cycle and significantly differed in reproduction ability among these populations. The reproduction of the RH1 population from Anthuriurn andraeanurn was significantly more than that of all other populations.

关 键 词：香蕉穿孔线虫 培养基 培养技术 繁殖力 

分 类 号：S432.45[农业科学—植物病理学]