野鸡源H9N2亚型禽流感病毒NA基因的序列分析  

Analysis of NA Gene of H9N2 Avian Influenza Virus from Pheasant

作　　者：罗琴芳[1] 刘华圆[1] 郭霄峰[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《华南农业大学学报》2006年第1期101-103,共3页Journal of South China Agricultural University

基　　金：国家自然科学基金(3027098730440010);广东省自然科学基金(020996)

摘　　要：通过病毒RNA抽提、RT-PCR、克隆,试验一次性获得野鸡源禽流感病毒A/Pheasant/Guangdong/GZI/2003(H9N2)NA全基因.序列分析及联机检索表明,该病毒的NA基因由1 458 bp组成,其中开放阅读框(ORF)1 401bp编码466个氨基酸;ORF与A/Ch icken/Guangdong/11/97同源性最高,达98%;与A/Ch icken/Guangdong/11/97的NA亲缘性最近.Based on the reported sequences of NA gene of avian influenza virus （AIV） H9N2 subtype, a pair of primers was designed for the amplification of NA gene from a strain of AIV H9N2 subtype isolated from a pheasant （A/Pheasant/Guangdong/GZI/2003（H9N2） by RT-PCR. Sequence analysis indicated that the NA gene was 1458 bp and the open reading frame （ORF） was 1401 bp in length, encoding for 466 amino acids, sharing more than 98% identities with A/Chicken/Guangdong/11/97（H9N2）.

关 键 词：禽流感病毒 NA基因 RT-PCR 克隆 序列分析 

分 类 号：S855.3[农业科学—临床兽医学]