不同培养液对昆明小鼠体外受精率及早期胚胎体外发育的影响  

Effects of Culture Media on Rate of Fertilization and Development of Early Embryos in Mice in vitro

作　　者：吴嫦丽[1] 张守全[1] 高淑静[1] 江青艳[1] 冯定远[1] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642

出　　处：《华南农业大学学报》2006年第1期107-109,共3页Journal of South China Agricultural University

基　　金：广东省自然科学基金(04020611);广东省教育厅自然科学基金(Z03005)

摘　　要：在TYH受精液中添加促卵泡素(FSH)和苯甲酸雌二醇(E2),检测不同浓度的FSH和E2对昆明小鼠的体外受精率的影响;检测在M16、CZB两种培养液中添加胎牛血清(FCS)与否对昆明小鼠早期胚胎体外发育的影响.结果表明:在TYH中添加1.0 IU/mL FSH,受精率和2-细胞胚率显著高于对照组;在TYH中添加0.1μg/mL E2,受精率和2-细胞胚率也显著高于对照组.在CZB中添加体积分数为10%FCS,可显著减少2-细胞期体外发育阻断,并显著提高小鼠胚胎体外发育的囊胚率,可见,以TYH添加1.0 IU/mL FSH和0.1μg/mL E2为受精液,以含体积分数为10%FCS的CZB为胚胎培养液,小鼠胚胎可获得较高的囊胚率.葡萄糖并不是小鼠胚胎形成囊胚所必需的.To determine whether efficiency of fertilization in vitro could be improved by estrogen （ E2 ） or follicle stimulating hormone （FSH）, different concentrations of E2 （0. 1, 0. 5 or 1.0 μg/mL）, or FSH （0. 1,0. 5, 1.0 IU/mL） were tested in the medium TYH. Kunming mouse early embryos were cultured in four media [M16,M16 ＋ 10% （φ） FCS,CZB,CZB ＋ 10% （φ） FCS] in vitro. The fertilization rate and 2-cell embryo rate were significantly higher in 1.0 IU/mL FSH and 0. 1 μg/mL E2 than controls. Two-cell embryo block of development in vitro was overcome and the blastocyst formation rate was higher in CZB ＋ 10% （φ） FCS than in other media. The results showed that glucose is unnecessary for the embryo to form blastocyst.

关 键 词：昆明小鼠 体外受精 早期胚胎 体外发育 

分 类 号：Q954.4[生物学—动物学]