Effects of Nitrobenzenes on DNA Damage in Germ Cells of Rats  

作　　者：XU Jing-bo JING Ti-song YANG Li SUN Zhi-wei SHI Long 

机构地区：[1]Department of Environmental Science, Northeast Normal University, Changchun 130024, P. R. China [2]College of Environment and Resource, Jilin University, Changchun 130026, P. R. China [3]Public Health College, Jilin University, Changchun 130021, P. R. China

出　　处：《Chemical Research in Chinese Universities》2006年第1期29-32,共4页高等学校化学研究（英文版）

基　　金：SupportedbyNationalNaturalScienceFoundationofChina(No.30370262),NaturalScienceFoundationofJilinProvince(No.20020633-2),NationalBasicResearchProgram(973Project)ofChina(No.2004CB418505)andSubjectConstructionofDoctoralDe-greebytheMinistryofEducationofChina(No.20040200002).

摘　　要：The single cell gel electrophoresis （SCGE） technique was used to detect DNA damage in germ cells of rats, which was induced by five tested nitroaromatic compounds. Mixed cultures of Sertoli and germ cells were prepared from the rats testis and their responses to rn-dinitrobenzene （ m-DNB）, 2,4-dinitrotoluene （ 2,4-DNT）, 2,6-dinitroto-luene（2,6-DNT）, 4-nitrotoluene（4-NT） and 2,4-dinitroaniline（2,4-DNAn） were studied. The results show that all the five chemicals have a reproductive toxicity. Each dose group and the control group were significantly different （ P 〈 0. 01 ）. All of them can lead to the damage to DNA in the germ cells of Kunming male rats in the definite range of concentration（m-DNB ： 0. 04-25μmol/L; 2,4-DNT, 2,6-DNT and 4-NT： 0. 032-500μmol/L; 2,4-DNAn ：0. 8-20μmol/L）. When the concentration increases, the damage rate will become higher, which shows an evident logarithm dose-effect relationship.The single cell gel electrophoresis （SCGE） technique was used to detect DNA damage in germ cells of rats, which was induced by five tested nitroaromatic compounds. Mixed cultures of Sertoli and germ cells were prepared from the rats testis and their responses to rn-dinitrobenzene （ m-DNB）, 2,4-dinitrotoluene （ 2,4-DNT）, 2,6-dinitroto-luene（2,6-DNT）, 4-nitrotoluene（4-NT） and 2,4-dinitroaniline（2,4-DNAn） were studied. The results show that all the five chemicals have a reproductive toxicity. Each dose group and the control group were significantly different （ P 〈 0. 01 ）. All of them can lead to the damage to DNA in the germ cells of Kunming male rats in the definite range of concentration（m-DNB ： 0. 04-25μmol/L; 2,4-DNT, 2,6-DNT and 4-NT： 0. 032-500μmol/L; 2,4-DNAn ：0. 8-20μmol/L）. When the concentration increases, the damage rate will become higher, which shows an evident logarithm dose-effect relationship.

关 键 词：Nitroaromatic compound Sertoli-germ cell coculture SCGE DNA damage 

分 类 号：Q954.43[生物学—动物学]