细胞凋亡及bax基因表达与舌苔变化关系的研究  

A Study on the Relationship Between the Gene Expression of Bax and Cell Apoptosis in Tongue Mycoderma Epithelium of Common Tongue Coating

作　　者：李新华[1] 吴正治 李明 张永锋 陈嫚茵 

机构地区：[1]湖南中医学院,湖南长沙410007 [2]深圳市中西医结合临床研究所,广东深圳518029

出　　处：《中医药学刊》2006年第1期57-59,共3页Study Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(39770890)

摘　　要：目的:检测常见舌苔舌上皮细胞凋亡情况及凋亡相关基因baxmRNA和蛋白产物,探讨舌苔厚度变化与舌上皮细胞凋亡、bax基因表达的关系。方法:运用TUNEL(末端脱氧核苷酸转移酶介导的脱氧尿嘧啶核苷三磷酸缺口末端标记)技术、原位杂交、免疫组化和图像分析技术。结果:与正常薄苔比较,剥苔bax基因过度表达伴随细胞凋亡增多,而厚苔bax基因低表达伴随细胞凋亡减少。bax基因表达水平变化趋势与细胞凋亡水平变化趋势一致。结论:bax基因表达水平的变化可能是影响舌苔上皮细胞凋亡并导致舌苔厚度变化的重要原因。Objective ： To investigate the relationship between the gene expression of bax and cell apoptosis in tongue mycoderma epithelium of common tongue coating. Methods ： TUNEL（ terminal deoxynucleotidyl transferase - mediated d - UTP nick - end labeling） technique, in stitu hybridization, immunohistocheraical technique and image analysis were used in the study. Results： In comparison with normal tongue coating,there was an over - expression of bax in peeling fur and accompanied with up - regulation of apoptosis , while there was an lower - expression of bax in thick coated tongue and accompanied with down - regulation of apoptosis. The change of bax gene expression level had the same tendency with that of cell apoptosis level. Conclusion ： The findings suggested that the change of expression level of bax effect cell apoptosis in tongue mycoderma epithelium , thus be one possible cause for the thickness＇s change of tongue coating.

关 键 词：舌苔 细胞凋亡 基因 BAX TUNEL技术 原位杂交 免疫组织化学 图像分析 

分 类 号：R2-03[医药卫生—中医学]