RP-HPLC法测定肾茶药材中迷迭香酸的含量  

Determination of Rosmarinic Acid in Clerodendranthus spicatus by RP- HPLC

作　　者：张继华[1] 王跃生[1] 柏健[1] 李霞兰[1] 何明珍[1] 杨世林[1] 

机构地区：[1]中药固体制剂制造技术国家工程研究中心,南昌330006

出　　处：《中药新药与临床药理》2006年第1期43-44,共2页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立高效液相色谱法测定肾茶药材中迷迭香酸含量的方法。方法采用反相高效液相色谱(RP-HPLC)法测定肾茶药材中迷迭香酸的含量,色谱柱:HypersilODS-C18柱(250mm×4.6mm,5μm);甲醇-0.1%磷酸水溶液(40∶60)为流动相;流速为1.0mL/min;紫外检测波长为280nm;柱温为25℃。结果该法可测定肾茶药材中迷迭香酸的含量,迷迭香酸在0.1076～0.5380μg的范围内与峰面积呈良好线性关系,平均回收率为98.54%,RSD=1.25%(n=6)。结论该方法简便、准确,可作为肾茶药材中迷迭香酸的含量测定方法。Objective To establish a HPLC method for the determination of rosmarinic acid in Clerodendranthus spicatus. Methods The determination was performed in Hypersil ODS - C18 column. The mobile phase was methanol - 0. 1% phosphoric acid water solution（40 ： 60） and UV detection wavelength was at 280 nm . The flow rate was 1.0 mL · min^-1 and the column temperature was at 25 ℃. Results The content of rosmarinie acid in Clerodendranthus spicatus could be determined by HPLC . There is a good linearity between the absorption peak area and the amount range of 0. 107 6 - 0. 538 0μg. The average recovery was 98.5 % . Conclusion This method is simple , accurate and can be used to determine the contents of rosmarinie acid in Clerodendranthus spicatus.

关 键 词：肾茶 迷迭香酸 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]