HPLC法测定莱葛颗粒中葛根素的含量  

Separation and Determination of Puerarin in Laige Granules by HPLC

作　　者：侯士果[1] 王书妍[1] 李洪霞[1] 柴金玲[1] 谷学新[1] 

机构地区：[1]首都师范大学化学系,北京100037

出　　处：《中药新药与临床药理》2006年第1期47-49,共3页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立测定莱葛颗粒中葛根素含量的反相高效液相色谱法。方法采用EconosphereC18柱(4.6mm×250mm,5μm);流动相为甲醇-1%醋酸水溶液(25∶75);流速0.8mL/min;检测波长250nm;柱温40℃。结果葛根素在0.2～1.0μg范围内与峰面积具有良好的线性关系,r=0.9997;加样回收率为100.56%,RSD为1.21%(n=5)。结论该方法简便、准确,灵敏度高,重现性好,可作为莱葛颗粒的质量控制方法之一。Objective To establish a RP - HPLC method for quantitative determination of puerarin in Laige Granules. Methods The separation was performed on Econosphere C18 column （4. 6 mm × 250 mm, 5μm） with mobile phase of methanol and 1% acetic acid solution （25 ： 75） at a flow rate of 0.8 mL/min. The UV detection wavelength was 250 nm and the column temperature was 40 ℃. Results The linear range of puerarin was 0. 2 - 1.0μg, r = 0. 999 7. The mean recovery was 100.56 % （RSD = 1.21% , n = 5 ）. Conclusion This method is simple, accurate, sensitive and with good reproducibility.

关 键 词：莱葛颗粒 葛根素 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]