微波辅助提取白僵蚕中的酪氨酸酶抑制剂  

Microwave-assisted extraction of tyrosinase inhibitor from batryticated silkworms

作　　者：龚盛昭[1] 杨卓如[1] 刘念平[2] 

机构地区：[1]华南理工大学化工与能源学院,广东广州510640 [2]广东化工制药职业技术学院化妆品系,广东广州510610

出　　处：《化学研究与应用》2006年第1期101-103,共3页Chemical Research and Application

基　　金：广东省佛山市产学研专项资金项目(2003A36)

摘　　要：The microwave-assisted extraction(MAE)technology of tyrosinase inhibitor from batryticated silkworms was studied with the yield and the relative inhibitory activity against tyrosinase(RIAAT) of the extract used as evaluating indexes in the experiment.The effects of extraction variables,especially solvent concentrations,microwave power,the duration of microwave irradiation,materials-solvent mass ratio and material particle size on extraction efficiency were investigated.It was concluded that the optimal condition was two doses of microwave radiation(450 W) for 7 min with a mass ratio of 70 % ethanol to material by 8∶1.The extract of batryticated silkworms with 15.8 % yield and 66.7 % RIAAT was obtained after the extracted liquor was decoloured with active char,concentrated and then dried under vacuum.Compared with direct-heating extraction(DHE),MAE can shorten extracting duration,improve the yield and RIAAT of the extract.The microwave - assisted extraction（MAE） technology of tyrosinase inhibitor from batryticated silkworms was studied with the yield and the relative inhibitory activity against tyrosinase （RIAAT） of the extract used as evaluating indexes in the experiment. The effects of extraction variables, especially solvent concentrations, microwave power, the duration of microwave irradiation, materials - solvent mass ratio and material particle size on extraction efficiency were investigated. It was concluded that the optimal condition was two doses of microwave radiation （450 W） for 7 min with a mass ratio of 70 % ethanol to material by 8： 1. The extract of batryticated silkworms with 15.8 % yield and 66.7 % RIAAT was obtained after the extracted liquor was decoloured with active char, concentrated and then dried under vacuum. Compared with direct -heating extraction （DHE）, MAE can shorten extracting duration, improve the yield and RIAAT of the extract.

关 键 词：白僵蚕 微波辅助提取 酪氨酸酶 中药 

分 类 号：O629.73[理学—有机化学]