E-选择素A561C(S128R)基因多态性对动态血压的影响  

The effect of E-selectin A561C (S128R) polymorphism on ambulatory blood pressure monitoring

作　　者：陈海翎[1] 华琦[1] 刘荣坤[1] 杨峥[1] 

机构地区：[1]首都医科大学宣武医院心内科,北京2002级博士研究生100053

出　　处：《心肺血管病杂志》2005年第4期196-200,共5页Journal of Cardiovascular and Pulmonary Diseases

基　　金：首都医科大学发展基金

摘　　要：目的:研究E-选择素A561C基因多态性对动态血压的影响.方法:原发性高血压组347例,对照组315例.2组年龄、体质指数(BMI)无显著性差异.对2组进行诊室血压测量,抽血检验生化指标.PCR-RFLP方法进行DNA多态性分析,电泳判断基因型并测序.结果:检测到AA、AC、CC三种基因型.在高血压组与非高血压组之间,基因型构成比有显著性差异(P<0.05).在高血压组AC-CC基因型和C等位基因频率(6.92%和4.76%)显著高于对照组(分别为3.17%,2.22%),P值分别为0.029和0.013.C等位基因发生高血压的风险性是A等位基因的2.197倍 [OR(Odds Ratio)=2.197,95%可信区间为1.164～4.144].同时AC-CC基因型的24 h、白天、夜间血压以及血压最大值、最小值、血压负荷均大于AA基因型,其中夜间舒张期血压和舒张期血压负荷的P值分别是0.043和0.016,具有统计学意义.而且AC-CC基因型发生收缩压非勺型高血压的危险是AA的4.367倍.OR=4.367,95%可信区间:0.047～1.122.发生舒张压非勺型高血压的危险是AA的1.972倍.OR=1.972,95%可信区间:0.124～2.070.结论:E-选择素A561C基因多态性对高血压有显著影响,AC_CC基因型和C等位基因发生高血压风险较大,动态血压各血压指标较高,且更易发生血压节律异常.Objective：To investigate the effect of E-selectin A561C （S128R） polymorphism on ambulatory blood pressure. Method： Genotypes of 347 essential hypertension patients[ male 163, female 184, age 46.08 ± 12.49, BMI[（26.13 ± 3.14）kg/m^2] and 315 healthy individuals [male 93,female 222,age 42,21 ± 14.67, BMI（25.66 ± 3.19]kg/m^2 were typed by polymerase chain reaction restriction fragment length polymorphism（PCR-RFLP）. Blood pressure and BG, TG, TC, LDL, HDL, UA, BUN of each indivktuals were examined. Result： Three genotypes （ AA, AC,CC） were found. In EH group, the percentage of genotype AC-CC（6.92%） and allele C （4.76%） are significantly higher than in healthy group （3.17% and 2.22% ）, P = 0. 029 and 0. 013. Risk of hypertension for allele C is 2.197 times of that for allele A（ OR = 2,197,95 % CI ： 1.164-4. 144）. 24 hSBP, 24 hDBP, dSBP, dDBP, nSBP,nDBP, MAXSBP, MAXDBP, MINSBP, MINDBP, LoadSBP and LoadDBP are higher in individuals with CC-AC genotypes,especially for nDBP and I,oadDBP（ P = 0.043 and 0.016）. Risk of nondipper SBP for AC-CC is 1.972 times of that for AA （OR=4.367,95% CI：0.047-1. 122） ;and risk of nondipper DBP for AC-CC is 4.367 times of that for AA OR = 1.972,95% CI：0.124-2.070）. Conclusion： E-selectin A561C （S128R） polymorphism affect blood pressure significantly

关 键 词：E-选择素 基因多态性 高血压 动态血压 

分 类 号：R541.4[医药卫生—心血管疾病] R587.1[医药卫生—内科学]