血小板生成素和转化生长因子-β_1在儿童血液病中的研究  

A study on thrombopoietin and transforming growth factor-beta1 in children with hematological diseases

作　　者：丁红芳[1] 高欣义[1] 杨昭华[1] 王连英[1] 刘杰[1] 

机构地区：[1]山东省胜利油田中心医院儿科,257034

出　　处：《中国小儿血液》2005年第6期255-257,共3页China Child Blood

摘　　要：目的探讨血液病儿童血清和骨髓上清液中TGF-β1、TPO的水平及其临床意义。方法采用ELISA法测定急性特发性血小板减少性紫癜、再生障碍性贫血、急性淋巴细胞白血病患儿血清及骨髓上清液中TGF-β1、TPO水平。结果ITP患儿血清TPO水平高于对照组,但差异无显著性(P>0.05),TGF-β1水平明显高于对照组(P<0.01),骨髓上清液中TPO和TGF-β1水平均高于对照组(P均<0.01);AA患儿血清和骨髓TPO水平显著增高,与健康对照组比较具有显著性差异(P<0.01),ALL患儿TPO水平虽低于AA组,但仍明显高于对照组(P<0.01);AA组血清TGF-β1水平与正常对照组比较无显著性差异,ALL患儿血清及骨髓上清液中TGF-β1水平较对照组显著降低(P<0.01),CR后血清中和骨髓中TGF-β1水平上升,CR前后TGF-β1水平比较具有显著性差异(P<0.01)。结论TGF-β1、TPO在儿童血液病的病理生理过程中发挥重要作用,动态检测TGF-β1水平可为ALL患儿病情评估、预后估计提供客观依据。Objetive To study the prognostic significance of thrombopoietin（ TPO）, transforming growth factor beta 1 （ FGF - β1 ） in children with hematological diseases. Methods TGF - β1 and TPO levels in the serum and bone marrow of children with ITP, AA, ALL were detected using enzyme - linked immunosorbent assay（ELISA）. Results The serum level of TPO in ITP children was higher than controis,but had no significant difference. The serum level of TGF - β1, in ITP children was much higher than controls （P 〈0.01 ） . The bone marrow level of TPO and TGF - β1 in ITP children was higher than controls （ P 〈0.01 ） . In AA children, the serum was higher than in normal and had significant difference （P 〈0.01 ） . In ALL children , the serum TPO was lower than in AA children but much higher than in normal （P 〈0.01 ） . In AA children, the serum TGF - β1 was higher than in normal with no significant difference （ P 〉 0.05 ） In ALL children, the serum TGF - β1 was lower than in normal （ P 〈 0.01 ） . The level of TGF - β1 in the serum and bone marrow of children with ALL would increase to nearly the normal level when remission occurred. The level of TGF - β1 after therapy was higher than before treatment and control（ P 〈 0.01 ）. Conclusion Dynamic changes in the serum and bone marrow of TGF - β1 and TPO can be used as an indicator of therapeutic effect as well as prognosis in children with hematological diseases.

关 键 词：血小板生成素 转化生长因子-Β1 儿童 血液病 儿童血液病 转化生长因子-Β1 血小板生成素 血清TGF-Β1 急性特发性血小板减少性紫癜 健康对照组 白血病患儿 显著性差异 再生障碍性贫血 

分 类 号：R725.5[医药卫生—儿科]