天山根瘤菌（Rhizobium　tianshanense）全细胞蛋白电泳和多位点酶电泳分析  

ANALYSIS ON RHIZOBIUM TIANSHANENSE BYWHOLE-CELL PROTEIN ELECTROPHORESIS AND MULIILOCUSENZYME ELECTROPHORESIS

作　　者：许晓东[1] 陈文新[1] 

机构地区：[1]北京农业大学生物学院,中国科学院微生物研究所

出　　处：《微生物学通报》1996年第3期131-135,共5页Microbiology China

基　　金：国家自然科学基金

摘　　要：用全细胞可溶性蛋白电泳和多位点酸电泳对１５株天山根瘤菌（Ｒｈｉｚｏｂｉｕｍ　ｔｉａｎｓｈａｎｅｎｓｅ）和其它１０株快慢生根瘤菌进行聚类分析，结果表明Ｒ．ｔｉａｎｓｈａｎｅｎｓｅ种内菌株关系密切而与其它种不同。分析时将全细胞蛋白电泳图谱分为２５４等份，根据每等份内条带的有无进行聚类，结果与数值分类基本一致，说明用该方法对全细胞蛋白电泳结果进行聚类可以作为根瘤菌的分群手段；用所有出现的１０９种迁移率对１７种多位点酶的电泳结果作聚类分析，得到与全细胞蛋白电泳相似的结果。Fifteen strains of Rhizobium tianshanense and ten representative strains of describedrhizobial species were analysed by electrophoresis of whole-cell proteins and multilocusenzyme. Electrophoretic profile of whole-cell proteins for each strain were divided into 254 equalparts. Distributions of protein bands in each part were recorded, and the date were used for clusteringanalysis. On the other hand, the relative migration of each multilocus enzyme band for each strainwere recorded. Total 109 migration values for 17 multilocus enzyme were obtained, and were also usedfor clustering analysis. The results that based on the date of these two electrophoretic analysis showedthat fifteen strains of R. tianshanense formed a unique group, differing from all described species. Thisconclusion was confirmed the results of phenotypic analysis, DNA-DNA hybridization, and partial16S rRNA sequencing. It suPPorted the proposal for R. tianshanense.

关 键 词：天山根瘤菌 全细胞蛋白电泳 多位点酶电泳 

分 类 号：Q939.114[生物学—微生物学]