氮源对钝齿棒杆菌谷氨酰胺合成酶的调节作用及溶菌酶对破壁作用的研究  

REGULATION OF NffROGEN SOURCES ON CORYNEBACTERIUM CRENATUM GLUTAMINE SYNTHETASE AND EFFECT OF LYSOZYME TREATMENT ON CELL DISRUPTION

作　　者：刘扬[1] 孔德刚[1] 高培基[1] 

机构地区：[1]山东大学微生物研究所

出　　处：《微生物学通报》1996年第3期150-154,共5页Microbiology China

摘　　要：钝齿棒杆菌（Ｃｏｒｙｎｅｂａｃｔｅｒｉｕｍｃｒｅｎａｔｕｍ）６２８２谷氨酰胺合成酶（ＧＳ）的合成显著受氮源性质的影响，以５０ｍｍｏｌ／Ｌ谷氨酸钠为唯一氮源时，谷氨酰胺合成酶活力最高；ＮＨ对谷氨酰胺合成酶的合成有阻遏作用，谷氨酸钠则具有解阻遏作用。蛋清溶菌酶对于菌株６２８２的细胞超声波破碎具有良好的辅助作用；适宜的细胞破碎条件为０．８ｍｇ／ｍｌ溶菌酶，３７℃保温４～５ｈ，超声波１２０Ｗ处理１２ｍｉｎ。The level of glutamine synthetsse in Corynebacterium crenatum6282 varied in responseto the nitrogen sources in cultllre medium. It was much higher in glutamate-grown cells than inammoniagrown cells. Ammonia can repress the formation of GS, and glutamate have thederepression effeCtion. Cell disruption of C. crenatum 6282 became much easier when lysozyme,extracted from egg white, was added to the cell suspicion. The suitable treatment conditionswas:added 0.8ing / ml lysozylne, incubated at 37t for 4～ 5h, then disrupted in a sonicator for 12min at 120W.

关 键 词：钝齿棒杆菌 谷氨酰胺合成酶 溶菌酶 氮源 

分 类 号：Q939.118[生物学—微生物学]