金银花中吡虫啉残留量的HPLC测定方法  

HPLC Determination of Imidacloprid Residue in Honeysuckle

作　　者：刘清浩[1] 刘红彦[1] 郭金春[2] 艾国民[1] 张玉军[3] 

机构地区：[1]河南省农业科学院植物保护研究所,河南郑州450002 [2]河南农业大学,河南郑州450002 [3]河南工业大学,河南郑州450052

出　　处：《河南农业科学》2006年第1期57-59,63,共4页Journal of Henan Agricultural Sciences

基　　金：国家科技攻关计划(2001BA701A58-01)

摘　　要：采用HPLC外标法测定金银花、土壤中吡虫啉的残留量。样品用甲醇提取,石油醚反萃取,二氯甲烷萃取,其中,金银花样品经弗罗里硅土与活性炭柱净化,使用ZORBAX SB C18柱,流动相为乙腈∶水(30∶70),流速0.8 ml/min,DAD检测器检测波长270 nm进行检测。采用该方法,吡虫啉回收率均大于80%,最低检出量为0.0212 ng,最低检出浓度为0.00265 mg/kg(花蕾)和0.001325 mg/kg(土壤)。A HPLC method was developed to determine imidacloprid residues in honeysuckle and soil. Samples were extracted by methanol, reversly purified by petroleum ether, cleaned up by dichloromethane. Furthermore, honeysuckle samples were cleaned up on a column of Florisil and activated carbon. The content of imidacloprid was determined by external standard method, ZORBAX SB C18 column was used with acetonitritLe and water solution （30：70,V： V） as mobile phrase at a flow rate of 0.8 ml/min, DAD detection wavelength at 270 nm. The recovery of more than 80 %, the lowest Limit of detectability of 0. 0212 ng, the lowest concentrations of detectability of 0. 00265 mg/kg （in flower） and 0. 001325 mg/kg （in soil） were obtained using this method.

关 键 词：金银花 吡虫啉 残留量 高效液相色谱 

分 类 号：S567.79[农业科学—中草药栽培]