同种带瓣大动脉超低温保存糖代谢测定及病理改变  

Change of the Sugar Metabolism Rate and Histological Study of Ca-yopreserve Valved Homograft Conduit

作　　者：段栩飞[1] 周诗琼[1] 鲁建生[1] 

机构地区：[1]武汉市儿童医院,湖北武汉430016

出　　处：《中华综合临床医学杂志（北京）》2006年第1期17-19,共3页chinese journal of composite clinical medicine

摘　　要：目的：探讨同种带瓣大动脉管道超低温（液氮）保存前、保存后不同时期血管的病理改变及糖代谢率变化。同时为能快捷、简便检测超低温（液氮）保存后同种带瓣大动脉管道生物活性，提供方法。方法：对13根同种带瓣大动脉根据保存时间不同分为3组：A组保存前、B组保存时间半年内、C组保存半年至1年。分别对3组进行病理切片检查、糖代谢率测定。病理学检查采用HE、PAS、Actin（SM）、Weigert氏法弹力纤维、Foot网状纤维染色以及吖啶橙内膜荧光染色等方法。其中HE染色（×400）采用微标卡尺测量内膜单位长度内内皮细胞剥脱长度。糖代谢率测定采用37℃孵化24小时，以Beckman全自动生化分析仪（过氧化物酶法）测定孵化前后培养液的糖浓度，求得组织糖代谢率（mM／g／ml／24h）。保存液为加入抗菌素的RPM11640小牛血清组织培养液。结果：1．同种带瓣大动脉保存前后糖代谢率（mM／g／ml／24h）结果：A组6．35±1．59、B组6．04±1．09、C组6．81±2．29。经统计学分析，差异无显著性（p〉0．05）；2．HE染色（×400）显示保存前后内皮细胞均有不同程度剥脱，采用微标卡尺测量单位长度内膜内皮细胞剥脱（um／cm）：A组369±134．36、B组701±417．20、C组350．14±298．09，经统计学分析，差异无显著性（p〉0．05）。3．PAS、Actin（SM）、Weigert氏法弹力纤维、Foot网状纤维染色显示中膜细胞结构及网状纤维完整；吖啶橙染色标本发出绿色荧光。结论：1．通过HE、PAS、Actin（SM）、Weigert’s弹力纤维、Foot网状纤维染色、吖啶橙荧光染色等病理学检查，证实超低温液氮保存前后，同种带瓣大动脉管道血管结构上无显著性改变。2．通过糖代谢率监测，证实超低温液氮保存前后，同种带瓣大动脉管道的糖代谢无显著性改变。3．HE、PAS、Actin（SM）、Weigert’s弹力纤维�Objective： To explore pathologic alteration and change of the sugar metabolism rate of allo-aorta before and after cryopreservation under liqiud nitrogen. To offer a rapid and convenient method to detect the biotic activity of allo-aorta by cryopreservation under liqiud nitrogen. Methods： Thirteen allo-aorta were chosen and divided into three groups. Before cryopreservation was group A.Having been preserved for half a year under liqiud nitrogen was group B. Having been preserved for half a year to a year was group C. Pathological section investigation and the sugar metabolism rate determination were adopted in there groups. Pathological investigation included HE,PAS, Actin（SM）,Weigert＇s, Foot＇s staining and acridine orange fluorescence（AOF） staining. Micro label rule was used to measure, length of stripping endothelium cell by HE staining（×400）. Beckman automatic biochemistry analyzer was used toinvestigate culture media sugar concentration before and after hatching 24 hours in 37℃.The cryopreservation medium was composed of RPMI1640,calf blood serum and antibioltic.Results：1.The sugar metabolism rate （mM/g/ml/24h）,group A 6.35±1.59,group B 6.04± 1.09, group C 6.81±2.29, difference was not significant by statistics analysis（p〉0.05）.2.Histologic modification 1 ：The cellular loss in the endounica （μm/cm）,group A 369±134.36,group B 701±417.20,group C 350.14±298.09,difference was not significant by statistics analysis（P〉0.05）.3. Cell structure and argyrophilic fiber of tuniea media were integrity by HE, PAS, Actin（SM）, Weigert＇s and Foot＇s staining. Stained preparation gave out green fluorescence by AOF staining. Conclusion： 1. Cryopreserve valved homograft conduit have no significant alteration before and after cryopreservation under liqiud nitrogen by Pathological investigation, such as HE, PAS, Actin （SM）,Weigert＇s,Foot＇s staining and AOF staining.2.The sugar metabolism rate of cryopreserve valved homograft conduit have no significant alterati

关 键 词：同种带瓣大动脉 液氮 保存 病理学 糖代谢 

分 类 号：R654.2[医药卫生—外科学] R644[医药卫生—临床医学]