香烟烟雾对大鼠脑血管细胞粘附分子-1表达的影响(英文)  

Effect of cigarette smoking on expression of vascular cell adhesion molecule-1 on cerebrovascular endothelial cells in rats

作　　者：孙芸[1] 柳忠兰[1] 

机构地区：[1]中国医科大学附属第一医院,辽宁沈阳110001

出　　处：《中国现代医学杂志》2006年第1期5-7,13,共4页China Journal of Modern Medicine

基　　金：Supported bygrants formHealthyMinistryNatural Science Foundation of China (98- 1- 196) and LiaoningScience and TechnologyFoundation of Chi-na. (9801500303)

摘　　要：目的为研究香烟烟雾对大鼠脑血管细胞粘附分子(VCAM-1)表达的影响,探讨吸烟致脑梗死的一种分子机制。方法建立大鼠吸烟模型,免疫组化方法检测大鼠脑血管VCAM-1表达。结果吸烟大鼠脑血管VCAM-1表达增加(大量吸烟组平均灰度116.08!2.38,小量吸烟组123.06!1.68,对照组130.33!1.88,即大量吸烟组血管内皮染色最深,VCAM-1表达最强,小量吸烟组次之,对照组最弱。经统计学分析,三组数值之间两两比较,差异均有显著性意义,P<0.05)。结论香烟烟雾使大鼠脑血管VCAM-1表达增加,脑血管内皮细胞VCAM-1表达增强可能是吸烟引起脑梗死的分子机制之一。[Objective] To study the effect of cigarette smoking on expression of VCAM-1 on cerebrovascular endothelium in rats for investigating one of molecular mechanisms of cerebral infarction induced by smoking. [ Methotis] Laboratory animals were grouped randomly into large-dose smoking, small-dose smoking and normal control. Then rats smoking models were made and immunohistochemical methods were used to detect the expression of VCAM-1 on cerebrovascular endothelial ceils in smoking rats. [Results] The cerebrovascular endothelial cells were all expressed with positive signal （brown granules） in all three groups, which was to say they were all expressed with VCAM-1 protein. By one way analysis of variance, two of three groups have marked statistical difference （P 〈0.05） separately on average gray value of VCAM-1 staining in vascular cellular endo the lium. And the mean gray value between large-dose and small-dose also included statistical difference. [Conclusions] Cigarette smoking can increase the expression of VCAM-1 in cerebrovascular endothelial cell; increasing expression of VCAM-1 in cerebrovascular cell may be one of the molecular mechanisms of cerebral infarction caused by smoking.

关 键 词：吸烟 脑血管内皮细胞 血管细胞粘附分子-1 大鼠 

分 类 号：R392.11[医药卫生—免疫学]