桂枝汤对低体温大鼠下丘脑组织蛋白激酶A和C活性的影响  

Effect of Guizhi Tang on the PKA and PKC Activities of Hypothalamus in Hypothermia Rats

作　　者：周军[1] 李沧海[1] 霍海如[1] 李兰芳[1] 康旭亮[1] 姜楠[1] 姜廷良[1] 

机构地区：[1]中国中医科学院中药研究所唐氏中药研究中心

出　　处：《中国实验方剂学杂志》2006年第1期20-22,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金重大研究计划资助(No:90209006)

摘　　要：目的:研究低体温大鼠下丘脑蛋白激酶A(PKA)、蛋白激酶C(PKC)活性的变化及桂枝汤的升温作用机理。方法:选用腹腔注射氨基比林低体温大鼠模型,先观测桂枝汤(p.o.)对模型大鼠体温的影响,继而采用放射性同位素法,测定给予桂枝汤后低体温大鼠下丘脑组织中PKA、PKC的活性。结果:桂枝汤可使低体温模型大鼠下丘脑组织中受抑的PKA、PKC活性显著增强,促进体温恢复。结论:PKA、PKC参与了大鼠的低体温过程,桂枝汤的升高体温的作用可能与提高下丘脑组织中PKA、PKC活性有关。Objective：To observe the changes of the activity of protein kinase A （PKA） and C （PKC）, and further investigate the mechanisms of anti-hypothermia action of Guizhi Tang. Methods： The hypothermia rats induced by i. p. aminopyrine were used to observe the action of Guizhi Tang on the body temperature and to detect the PKA and PKC activity in hypothalamus in model and Guizhi Tang-treated groups using isotopic tracing assay. Results：The drop of body temperature in rats induced by aminopyrine was counteracted and the PKA and PKC activities increased significantly after oral administration of Guizhi Tang. Conclusions： Both PKA and PKC may participate in the mechanism of hypothermia induction and the increases of PKA and PKC may contribute to the anti-hvoothermia action of Guizhi Tang.

关 键 词：低体温 桂枝汤 下丘脑 蛋白激酶A 蛋白激酶C 

分 类 号：R285.5[医药卫生—中药学]