人参中皂苷类化合物的HPLC-ESIMS研究  

Determination of Ginsenosides in Extract of Panax Ginseng by Liquid Chromatography-Electrospray Ionization Mass Spectrometry

作　　者：李丽[1] 刘志强[1] 许庆轩[1] 宋凤瑞[1] 刘淑莹[1] 

机构地区：[1]中国科学院长春应用化学研究所长春质谱中心,吉林长春130022

出　　处：《分析测试学报》2006年第1期45-48,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20173057);中国科学院知识创新工程重要方向项目(KGCX2-SW-213-06);吉林省科技发展计划重大项目资助项目(20011109)

摘　　要：利用高效液相色谱与电喷雾质谱联用技术分析鉴定了人参粗提物中人参皂苷类化合物。实验采用反相C18色谱柱,二元线性梯度洗脱(0.2%的醋酸水溶液和乙腈),分离并检测了人参中的皂苷类化合物;通过与电喷雾质谱的联用和质谱的源内C ID技术获得了其中主要色谱峰相对应化合物的相对分子质量和结构信息。A high performance liquid chromatography -electrospray ionization mass spectrometry method was developed for the analysis of ginsenosides in crude extracts from Panax ginseng. The analysis was performed on a reversed-phase C18 column using a binary eluent（0. 2% aqueous CH3COOH and acetonitrile） under gradient conditions. Seven major peaks were detected in the negative ion mode. The molecular [ M - H ] - ions of ginsenosides were observed, from which the relative molecular mass were obtained. A collision induced dissociation（CID） experiment was carried out in order to determine the structure of the ginsenosides. The LC - ESI MS method was particularly useful in the identification of some saponin isomers.

关 键 词：人参 皂苷类化合物 高效液相色谱-电喷雾质谱联用 源内CID质谱技术 

分 类 号：O657.14[理学—分析化学]