双胶束双手性添加剂电动毛细管色谱法手性分离西替利嗪及其中间体  

Enantioseparation of Cetirizine and Its Intermediates by Dual-micellar Electrokinetic Capillary Chromatography

作　　者：杜国华[1] 朱丽玢[1] 谢剑炜[1] 仲伯华[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《分析测试学报》2006年第1期57-60,共4页Journal of Instrumental Analysis

摘　　要：采用β-环糊精(-βCD)和牛磺去氧胆酸(TDCA)为手性添加剂,建立了西替利嗪及其中间体的双胶束双手性添加剂电动毛细管色谱对映体拆分方法,详细研究了影响对映体分离的各种因素,其中运行液pH值、TDCA、β-CD和十二烷基硫酸钠(SDS)的浓度对手性分离起着重要的作用。通过比较研究5种类型环糊精对手性分离的影响,结果表明,仅有β-CD能同时基线分离西替利嗪及其中间体。实验确定的最佳条件为:80mmol/L硼酸盐和90 mmol/L磷酸盐缓冲液,pH=5.6,含有12 mmol/L TDCA,9 mmol/L-βCD,2.1 mmol/LSDS。同时对分离机理也进行了探讨。Cetirizine and its intermediates were enantioseparated by dual-micellar electrokinetic capillary chromatography usingβ-cyclodextrin（β-CD） and taurodeoxycholic acid（TDCA） as selectors. The influences of the concentrations of TDCA, sodium dodecyl sulfate （SDS） andβ-CD, theβ-CD types and pH etc. on the retention time and enantioresolution were investigated to optimize separation conditions. Good separation of enantiomers of eetirizine and its intermediates was achieved in 80 mmol/L borate and 90 mmol/L phosphate buffer system（pH = 5.6） including 12 mmol/L TDCA, 9 mmol/Lβ- CD, and 2. 1 mmol/L SDS. The mechanism of chiral separation was also discussed.

关 键 词：胶束电动毛细管色谱法 西替利嗪 对映体分离 

分 类 号：O657.8[理学—分析化学]