Pb^(2+)与Co(phen)_3^(3+)及DNA间的相互作用及其分析测定的研究  

Electrochemical Studies on the Interactions among Pb^(2+), Co(phen)_3^(3+) and DNA

作　　者：陆宝仪[1] 李红[1] 

机构地区：[1]华南师范大学化学系,广东广州510631

出　　处：《分析测试学报》2006年第1期94-97,101,共5页Journal of Instrumental Analysis

基　　金：广东省教育厅自然科学基金(Z03020);广州市科技计划项目(2004J1-C0091)

摘　　要：用吸附干燥法制备了dsDNA/Au和Co(phen)33+-dsDNA/Au修饰金电极,采用循环伏安法、微分脉冲伏安法研究了Pb2+对Co(phen)33+和Co(phen)33+与DNA作用产物的伏安行为的影响,并对微量Pb2+的含量进行了测定。最后应用紫外光谱法进一步证实Pb2+与dsDNA及Co(phen)33+-dsDNA作用产物间存在明显的相互作用。The dsDNA/Au and Co（phen） 3^3 ＋ -dsDNA/Au modified gold electrodes were prepared by using the adsorptive dry method. The effects of Pb^2＋ on the voltammetric behavior of Co （phen）3^3＋ and the interactive product of Co（phen）3^3＋ with DNA were investigated by cyclic vohammetry and differential pulse vohammetry, In addition, the existences of the interaction of Pb^2＋ with dsDNA and with Co（phen）3^3＋ -dsDNA complex were further confirmed by UV spectrophotometry.

关 键 词：铅离子 DNA Co(phen)3^3+ 相互作用 循环伏安法 微分脉冲伏安法 

分 类 号：O657.11[理学—分析化学]