电化学发光淬灭法测定SOD  

Determination of SOD by Electrochemiluminescence Quenching Analysis

作　　者：储海虹[1] 吴莹[1] 狄俊伟[1] 屠一锋[1] 

机构地区：[1]苏州大学化学化工学院,江苏苏州215006

出　　处：《分析测试学报》2006年第1期125-126,129,共3页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20275025)

摘　　要：在硼酸缓冲溶液(pH 6.7)中性介质中,O2和H2O2均对鲁米诺的电化学发光(ECL)有敏化作用,而超氧化物歧化酶(SOD)对此敏化的电化学发光具有淬灭作用,SOD的浓度与两种发光体系的ECL光强成线性关系,可用于SOD的测定,为SOD的测定提供了一种灵敏、可靠的测定方法。It was found that the sensitized luminol electrochemiluminescence（ECL） formed through the reaction of dissolved oxygen or hydrogen peroxide With luminol at a boric acid buffer solution of pH 6.7, could be quenched by SOD. A sensitive and reliable method based on the above phenomenon was thus established for the determination of SOD. The sensitized ECL intensity has a linear relationship with the SOD concentration in the range of 0.24 -9.6 IU/mL for luminol -O2 system, and in the range of 0. 024- 1.8 IU/mL for luminol- H2O2 system. The mechanism of the quenching effect of SOD on the sensitized luminol ECL was also studied.

关 键 词：鲁米诺 电化学发光 超氧化物歧化酶 淬灭 

分 类 号：O657.1[理学—分析化学] Q554[理学—化学]