利用ISSR标记分析兴安落叶松种源的遗传多样性  

Analysis on the Genetic Diversity of Larix gmelinii Provenance by Using ISSR Markers

作　　者：那冬晨[1] 杨传平[1] 姜静[1] 夏德安[1] 

机构地区：[1]东北林业大学,黑龙江哈尔滨150040

出　　处：《林业科技》2006年第1期1-4,共4页Forestry Science & Technology

摘　　要：利用ISSR标记对17个兴安落叶松种源170个个体遗传多样性进行了分析。通过10个ISSR引物扩增。共检测到95个位点，各种源多态位点百分率在25．26％～44．21％之间，多态位点百分率最高的为鹤北种源，最低的为绰尔种源。根据种源间的遗传距离，构建了兴安落叶松17个种源的遗传聚类图。同时，结合遗传距离和地理距离对兴安落叶松进行了种源区划。Genetic diversity among 17 Larix gmelinii provenances, including 170 individuals, was detected using ISSR markers. 10 ISSR- primers were able to generate 95 loci in total. The differentiation of the percentage of polymorphic loci （PPL） among different provenances was evident, ranging from 25.26 % -44.21%, which Hebei provenance with the highest PPL and Chao＇er provenance with the lowest PPL. The dendrogram of genetic relation was comtrueted on the basis of the genetic distances among 17 provenances.At the same time, the proverauace division was completed according to genetic distances and geographic disances.

关 键 词：兴安落叶松 种源 ISSR 遗传多样性 

分 类 号：S791.222[农业科学—林木遗传育种] Q347[农业科学—林学]