癌胚抗原基因重组疫苗的构建及免疫效果观察  

The construction of recombinant CEA gene vaccine and its immune effect

作　　者：熊伟[1] 段秀梅[2] 方艳秋[2] 刘力华[2] 许淑芳[2] 姜艳芳[2] 谭岩[2] 

机构地区：[1]军事医学科学院微生物流行病研究所微生物检测研究中心,北京100071 [2]吉林大学第一医院中心实验室,长春130021

出　　处：《中国免疫学杂志》2006年第1期61-64,共4页Chinese Journal of Immunology

基　　金：吉林省科技厅重点项目(20050402-3;20040401-5)吉林省科技厅国际合作项目(20040707-2)2004年长春市科技局计划项目(2004218)高等学校博士学科点专项科研基金(20020183017)资助项目

摘　　要：目的:探讨含癌胚抗原(CEA)部分编码基因的真核重组质粒pIRES-CEAⅢ用于CEA阳性肿瘤免疫治疗可能性。方法:应用基因重组技术构建了含CEA信号肽和Ⅲ区编码序列的真核表达质粒pIRES-CEAⅢ,肌注免疫BALB/c小鼠, 再分别应用野生型小鼠肝癌细胞H22及CEA cDNA全序列转染的H22细胞进行小鼠皮下接种,观察基因重组疫苗对CEA阳性肿瘤的抑制作用及其诱导小鼠细胞毒性T淋巴细胞(CTL)特异性杀伤CEA阳性肿瘤细胞的能力。结果:pIRES-CEAⅢ真核重组质粒免疫组小鼠CEA阳性肿瘤的生长速度趋缓,瘤块较小,与对照组小鼠(免疫空载体质粒和接种野生型H22细胞)相比有明显差异(P<0．05);经pIRES-CEAⅢ重组质粒免疫的小鼠脾细胞对H22-CEA(+)细胞的杀伤率明显升高,差异显著(P< 0．01);但对H22细胞则没有明显的杀伤作用。结论:pIRES-CEAⅢ作为基因疫苗可以抑制CEA阳性肿瘤在小鼠体内的生长, 诱导小鼠CTL对CEA阳性肿瘤细胞的特异性杀伤。Objective：To investigate the possibility that the eukaryotic expression plasmid pIRES-CEAⅢ was used to therapy tumor expression CEA. Methods：The coding sequence for signal peptide and the third domain of CEA was inserted into the eukaryotic expression plasmid pIRES-neo using gene recombinant technique. BALB/c mice were immunized intramuscularly with recombinant plas- mid vaccine and inoculated sc by the wildtype mice hepatocellular carcinoma cell line of H22 and H22 transfected with plasmid containing the full sequence of CEAcDNA. Tumor sizes were observed and the specific CTL cytotoxicity of the mice was detected. Results： Compared with the control, the tumors in mice immunized with pIRES-CEAⅢ were significantly inhibited （P 〈 0. 05 ）. The splenic cells of mice immunized with pinES-CEAⅢ possess strongly the cytotoxicity against tumor H22-CEA（ ＋ ）（ P 〈 0. 05 or P 〈 0. 01 ）. Conclusion：pIRES-CEAⅢ vaccine can suppress the growth of tumor expressing CEA and elicit CTL response against CEA antigen.

关 键 词：癌胚抗原 基因疫苗 免疫治疗 

分 类 号：R392.11[医药卫生—免疫学]