HPLC法测定桂枝茯苓胶囊中肉桂酸、桂皮醛和丹皮酚的含量  

Determination of cinnamic acid,cinnamaldehyde and paeonol in Guizhifuling capsules by HPLC

作　　者：华剑[1] 武超[1] 

机构地区：[1]蚌埠医学院附属医院,安徽蚌埠233004

出　　处：《安徽医药》2006年第1期30-31,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的 测定成药桂枝茯苓胶囊中肉桂酸、桂皮醛和丹皮酚的含量。方法 用HPLC法，Shim-packCLC-ODS色谱柱为固定相，乙腈-0．1％磷酸溶液（30：70）为流动相；检测波长为284nm。结果 肉桂酸的线性范围为0．42～2．52mg·L^-1（r=0．9998），桂皮醛的线性范围为3．60～36．00mg·L^-1（r=0．9998）和丹皮酚的线性范围为6．05～60．5mg·L^-1（r=0．9999）。平均回收率肉桂酸为98．2l％（RSD=2．64％，n=5），桂皮醛为96＋28％（RSD=1．40％，n=5），丹皮酚为98．78％（RSD=1．49％．n=5）。结论 此方法快速、准确、适用于桂枝茯苓胶囊的含量测定，且所测三种主要成分之间无于扰。Aim To determine cinnamaldehyde, cinnamic acid and paeonol in Guizhifuling capsules. Methods HPLC method was used. The samples were separated on a Shim-pack CLC-ODS column with the mobile phase of acetonitrile ：0.1% phosphoric acid （30： 70） and the detection wavelength was set at 284 nm. Results The calibration curves were linear in the range of 0.42 ～ 2.52 mg· L^-1 in cinnamic acid （ r = 0.9998 ）, 3.60 ～ 36.00 mg · L i in cinnamaldehyde （ r = 0. 999 8 ） and 6.05 ～ 60.5 mg· L^-1 in paeonol （ r = 0.999 9 ） respectively. The average recoveries of cinnamic acid in Guizhifuling capsules were 98.21% （ RSD = 2.64%, n = 5 ）, and of cinnamaldehyde were 96. 28% （RSD=l.40%,n=5）,andofpaeonolwere98.78% （RSD=l.49%,n=5）respectively. Conclusion Themethod is quick, accurate and suitable for the determination of Guizhifuling capsules. There is noninterference among the three main contents in Guizhifuling capsules.

关 键 词：HPLC 桂枝茯苓胶囊 肉桂酸 桂皮醛 丹皮酚 

分 类 号：R286.0[医药卫生—中药学]