丙泊酚对缺氧大鼠海马脑片诱发电位的影响  

The effects of propofol on the evoked potential in hypoxic hippocampal slices of rat

作　　者：张邓新[1] 曾因明[2] 江山[2] 丁浩中[1] 张焰[1] 

机构地区：[1]无锡市第四人民医院麻醉科,江苏无锡214062 [2]徐州医学院江苏省麻醉学重点实验室,江苏徐州221002

出　　处：《徐州医学院学报》2006年第1期23-26,共4页Acta Academiae Medicinae Xuzhou

摘　　要：目的采用离体海马脑片缺氧模型,观察丙泊酚对缺氧大鼠海马脑片诱发电位的影响。方法用细胞外记录方法,电极刺激海马CA3区谢弗侧支,在CAl锥体细胞层记录诱发电位。观察丙泊酚对缺氧海马脑片诱发电位———顺向群峰电位(OPS)和缺氧损伤电位(HIP)的影响。大鼠海马脑片随机分为4组,每组8个脑片。缺氧组:脑片用缺氧无糖的人工脑脊液(ACSFOGD)灌流14 min,实施缺氧过程;药物组分为P1、P5和P15组:在ACSFOGD灌流液中分别含1,5和15μmol.L-1的丙泊酚。结果缺氧组OPS消失时间为(186±26)s,在缺氧10 min左右,均出现HIP,持续时间为(113±16)s;恢复供氧后,OPS恢复率和恢复幅度分别为25%和(30±23)%。与缺氧组相比,P5及P15组OPS消失时间向后推迟,OPS恢复率和恢复幅度都有增加;并且HIP出现率下降,其出现时间明显延后、持续时间显著增加。而P1组各项指标与缺氧组相比,无明显差异。结论丙泊酚能改善缺氧海马脑片细胞外记录电生理指标,提示对脑缺氧损伤有保护作用。Objective To evaluate the effects of propofol on the extracellular evoked potential in hypoxic hippoeampal slices of rat. Methods The evoked potential of pyramidal cells in CA1 region of the hippocampal slice was recorded by using extracellular recording technique, while the Schaffer collateral fibers in the CA3 region were stimulated. The effect of propofol on the evoked potential, in terms of orthodromic population spike （OPS） and hypoxia injury potential （HIP）, were investigated in the ischemic hippocampal slices. The rat hippocampal slices were randomly divided into four groups （ n = 8 each）. The process of cerebral hypoxia was simulated by giving superfusion with ACSFOGD for 14 minutes in the control and test groups. The propofol test group was subdivided into P1, P5 and P15 groups to undergo superinfusion with ACsFooD containing 1, 5 and 15μmol·L^-1 propofol respectively. Results In hypoxia control group, the time for OPS to disappear was （ 186 ±16） seconds; HIP occurred in all slices within about 10 minutes of hypoxia and lasted for （113±16） seconds. When normal ACSF superfusion was restored, the rates of OPS recovery and OPS amplitude were 25% and （30±23） % respectively. Compared to that in the hypoxia control group, the disappearance of OPS in the P5 and P15 groups was postponed, and the recovery rote and recovery amplitude of OPS were higher after the restoration of oxygen supply, and additionally, HIP occurred late and lasted longer. No significant differences were found between P1 and hypoxia control groups in OPS and HIP. Conclusion Propofol could improve some electrophysiologic indexes of hypoxic hippocampal slices, suggesting that propofol can offer protective effects against brain hypoxic injury.

关 键 词：海马 缺氧 顺向群峰电位 缺氧损伤电位 丙泊酚 

分 类 号：R961[医药卫生—药理学] R971.2[医药卫生—药学]