聚羟基烷酸酯薄膜的亲水性改性  

Hvdrophilicity Improvement of Polyhydroxyalkanoate Films

作　　者：卢玲[1] 王迎军[1] 郑裕东[1] 吴刚[1] 

机构地区：[1]华南理工大学材料学院生物材料研究所特种功能材料及其制备新技术教育部重点实验室,广州510641

出　　处：《应用化学》2006年第1期22-25,共4页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金资助项目(50272021;59932050);"八六三"项目(2003AA205053);中国博士后科学基金资助项目(2003033035);广东省十五重大科技专项资助项目(A0302020202;2003A1070102);广东省自然科学基金博士科研启动基金资助项目(04300428);华南理工大学自然科学基金项目(E5324298)

摘　　要：分别采用氧等离子体方法和碱性条件下水解的方法对新型生物高分子3-羟基丁酸和3-羟基戊酸共聚物(PHBV)薄膜进行改性处理,以改善其亲水性和细胞亲和性。采用XPS考察了改性前后薄膜表面的组成、元素化学状态,用SEM观察了表面形貌变化,并考察了犬骨髓基质细胞在其上的生长情况。结果表明,经等离子体和水解改性后PHBV薄膜表面的氧含量分别增加了5．3％和2．5％,水接触角分别减小了36°和 16．7°,表明材料表面的亲水性增强。在氧等离子体处理后分别有1．5％和3．5％的C-O和C-C发生断裂, 生成亲水的羧基,而在碱性溶液中则发生酯键的水解。改性后的PHBV薄膜更有利于细胞的生长,尤以采用氧等离子体处理的效果更为明显。研究结果为进一步的表面改性修饰以及制备细胞亲和性好的PHBV三维支架打下良好基础。New biopolymer poly （3-hydroxybutylate-co-hydroxyvalerate） （PHBV） films were treated with oxygen plasma and hydrolyzed under alkaline condition to render them hydrophilicity and cell affinity. The surface composition and elemental chemical state of PHBV films after modification were characterized via XPS, while surface morphologies were observed with SEM, and the growth of bone marrow stromal cells（BMSCs） on the films were also investigated. It was shown that oxygen contents of PHBV films were increased by 5.3% and 2.5%, water contact angles were reduced by 36° and 16.7° respectively after oxygen plasma treatment and hydrolysis, the results suggested the improvement of surface hydrophilicity. After oxygen plasma treatment, 1.5% C-O bonds and 3.5% C-C bonds cleft and carboxyl bonds were generated, while under alkaline condition the ester bonds in PHBV hydrolyzed to form more hydroxyl and carboxyl bonds. Modified PHBV films were favourable for cell growth, especially the films after oxygen plasma treatment. The results have laid the foundation for further surface modification and preparation of three-dimensional PHBV scaffold with good cell affinity.

关 键 词：PHBV 氧等离子体 水解 亲水性 

分 类 号：O631.3[理学—高分子化学] TQ323.4[理学—化学]